Method for domesticating and separating electricigens by electrochemistry

Abstract

The invention relates to a method for acclimating and separating electricigens by electrochemistry and high efficient electrogenesis flora obtained in the method. The method is realized mainly by electrochemistry of a microbial battery, and a great deal of high efficient electrogenesis microorganism can be enriched by repeatedly acclimating and selecting mixed microorganism in inoculation liquid (industrial sewage, domestic sewage and the like); meanwhile, in the process of acclimating the microorganism, the electrogenesis capacity of the battery and the degradation for anode liquor COD can be promoted. The method is simple in operation, is beneficial to screening the high efficient electrogenesis microorganism and realizing the joint operation of acclimating and separating technology and battery running, and overcomes the defect that the target strain is difficult to obtain in the traditional direct separation technology (plate streaking separation technology and the like); furthermore, the complicated operation of a repeatedly scraping membrane acclimation method and the defects that the operation cycle is long and microorganism electrogenesis activity is influenced can be avoided.

Description

technical field [0001] The present invention relates to a method for domesticating electricity-producing microorganisms in a microbial battery inoculum (industrial wastewater, domestic sewage, etc.) by an electrochemical method, the purebred high-efficiency electricity-producing bacteria obtained after domestication and separation by the method, and the microbial battery thereof application. Background technique [0002] Due to the increasing shortage of energy, the development of new clean energy and the adjustment of energy structure have become the consensus of the world. In this context, bioelectrochemical technology is widely used, including microbial fuel cells (MFC) Electrolysis cell (MEC) technology, the former uses microorganisms as catalysts to convert the chemical energy stored in biomass into electrical energy through the metabolism of microorganisms; the latter uses microorganisms as catalysts, with the assistance of external voltage A device that converts chem...

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Problems solved by technology

There is no clear standard for this kind of domestication and separation operation to judge the maturity of the microbial film, and the scraping operation will cause the loss of membrane activity and prolong the time required for membrane re-maturation. 63 days, the operation period is long, the operation is complicated, and the anaerobic conditions are very strict in the whole process. The feasibility of this method in the industrial application of MFC and MEC is extremely low

In addition, the screening method for electrogenic microorganisms mentioned in the domestic patent "A Device for High-throughput Cultivation and Screening of Electrogenic Microorganisms" (Patent No.: 200810114729.6) focuses on several kinds of electrogenic microorganisms that have obtained electrogenic activity. comparison, so as to select microorganisms with greater electricity-producing activity, and the installation of the screening device is complicated, and the operating environment of the device is strict, which is not suitable for the industrialized screening process of electricity-producing microorganisms

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