Method for inducing bees to pollinate among parents of male sterile line seed

A parent and bee technology, applied in the fields of botanical equipment and methods, plant genetic improvement, animal feed, etc., can solve the problems of unsatisfactory effect and low hybrid seed yield, and achieve effective pollination time shortening, significant economic benefits, strong The effect of operability and practicality

Inactive Publication Date: 2011-11-02
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the collection behavior of bees is very specific, that is, they only visit flowers with the same genetic background during a specific period of time every day, and the genetic backgrounds of male and female parents of male sterile lines are often different, so that bees Only tend to visit the male parent that has both pollen and nectar, so the effect of cross-pollination between parents is not very satisfactory, and the yield of hybrid seeds is not high

Method used

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  • Method for inducing bees to pollinate among parents of male sterile line seed
  • Method for inducing bees to pollinate among parents of male sterile line seed
  • Method for inducing bees to pollinate among parents of male sterile line seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 - Using quercetin to stimulate honeybees to pollinate the hybrid seed production of sterile cauliflower lines

[0034] 1 material

[0035] The parent is code 3592. The parent is provided by the Economic Crops Research Institute of Yunnan Academy of Agricultural Sciences, and the female parent is a cytoplasmic male sterile line.

[0036] 2 Experimental Design

[0037] The experiment was carried out in the vegetable breeding base in Yuanmou County, Yunnan Province. A plot with an area of ​​1 mu was selected for the experiment, and the ratio of male and female parent was 1:4, respectively, which was an alternate planting (planting 1 row of duplicates and 4 rows of female parents). The test plots are all isolated from the outside world by gauze greenhouses (3 meters high).

[0038] On February 10, 3 boxes / mu of bees were released, with 8,000 to 10,000 bees per box. One group was fed pure syrup, and the other two groups were fed syrup containing quercetin, the...

Embodiment 2

[0047] Example 1 was repeated with the following differences: The concentration of quercetin was 0.10%. The results are shown in Tables 1 and 2.

Embodiment 3

[0049] Example 1 was repeated with the following differences: the secondary metabolite added to the syrup was caffeine, and the concentration of caffeine was 0.05%. The results are shown in Tables 1 and 2.

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Abstract

The invention discloses a method for inducing bees to pollinate among parents of male sterile line seed, comprising the following steps of: adding 0.05-0.1 part of plant secondary metabolites into syrup for feeding the bees; and feeding bee colonies which pollinates. Feeding at a time each day, wherein, feeding time is from 18:00 to 20:00 PM, and feeding quantity is 100-200 mg / bee colony / day. Feeding is continued until the end of florescence, and the pollinating bee colonies are moved out of a big-arch shelter. The method can stimulate the bee colonies to firstly pay a visit to male parents and carry the pollen of the male parents to pay a visit to female parents, so as to realize the cross pollination among the parents. The induction function has no specificity, namely, any one of compounds has the function for stimulating the bees to crossly pollinate among the parents. The invention is not limited by the cross pollination between special crops and special parents, has simple method, obvious economic benefit and wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of crop pollination, and relates to a method for inducing honeybees to cross-pollinate crops. In particular, it relates to a method for inducing honeybees to pollinate between parents of sterile lines. Background technique [0002] With a vast territory and favorable climatic conditions, my country has become the main base for crop seed production. In terms of crop seed production, the seed production technology using male sterile lines is relatively mature. Many high-yield and high-quality crops have been cultivated through male sterile lines, which have made great contributions to the development of the world economy. However, in the process of producing male sterile lines, the hybridization between parents is mainly achieved by artificial pollination, that is, the pollen of the male parent is collected manually, and the pollen is smeared on the stigma of the female parent. This is a very time-consuming...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01K67/033A23K1/18A23K1/16A23K20/00A23K20/163A23K50/90
Inventor 刘方邻李建军
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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