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Nucleic acid sequences of novel wheat potassium channel beta subunit KTB and KHB genes and the application thereof

A nucleic acid sequence and potassium channel technology, which is applied in the field of nucleic acid sequences of novel wheat potassium channel genes, and can solve problems such as publication.

Inactive Publication Date: 2009-10-28
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no relevant reports have been published in domestic and foreign publications

Method used

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  • Nucleic acid sequences of novel wheat potassium channel beta subunit KTB and KHB genes and the application thereof
  • Nucleic acid sequences of novel wheat potassium channel beta subunit KTB and KHB genes and the application thereof
  • Nucleic acid sequences of novel wheat potassium channel beta subunit KTB and KHB genes and the application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] 1. Material induction

[0109] Select one part of salt-tolerant and salt-sensitive barley and materials screened by our laboratory. Take 10 seeds of each material and put them in a petri dish lined with filter paper, add water and put them in a dark room to germinate. After two days, transfer them to flower pots filled with vermiculite for cultivation, still using water as the culture medium, light and dark 14 / 10 Hours, the daytime temperature is 22-25°C, and the nighttime temperature is 18-20°C, alternately culture until 3 leaves and 1 heart stage, change the MS culture medium to adapt to the culture for two days, and continue the culture with low-potassium and high-sodium MS culture medium (LK+MS) , and change the culture medium every 2 days. One week later, the roots were taken to extract total RNA.

[0110] In a large amount of LK+MS mother liquid, ammonium nitrate and ammonium dihydrogen phosphate are used instead of potassium nitrate and potassium dihydrogen pho...

Embodiment 2

[0188] 1. Material induction

[0189] One of the salt-tolerant and salt-sensitive wheat materials screened by our laboratory was selected for induction. The induction method is the same as the first step of Example 1.

[0190] 2. Total RNA extraction and reverse transcription

[0191] With the 2nd step of embodiment 1.

[0192] 3. PCR amplification primers

[0193] The following primers were designed according to the relevant EST sequences:

[0194] wheat:

[0195] f 1 (wheat): 5'-ATGCAGTACAACAACCTGGG-3';

[0196] R 1 (wheat): 5'-CCGCTTCATCTGTACGACTC-3'.

[0197] 4. PCR amplification

[0198] PCR reaction composition:

[0199] cDNA 200-300ng

[0200] dNTPs 200μM

[0201] 10*Taq Plus Buffer 5μl

[0202] Primer F 1 10pmol

[0203] Primer R 1 10pmol

[0204] Taq Plus Polymerase 1U

[0205] wxya 20 to a final volume of 50 μl

[0206] PCR reaction program:

[0207] (1) 94°C 5min

[0208] (2) 94℃ 45sec

[0209] (3) 63℃ 45sec

[0210]...

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Abstract

The invention discloses a novel wheat voltage gated potassium channel beta subunit encoding genes KTB and KHB (respectively from wheat and barley). Shown in sequence tables SEQ ID NO.1 and SEQ ID NO.2, the wheat crops absorb and transfer potassium ions in the different culture process, which can be evaluated through the gene sequences, and expression products can be used for presuming association of the potassium ions in an absorbing and transferring channel and related function substances. Meanwhile, the genes are transferred into the wheat crops by utilizing a transgene technology so as to obviously improve low potassium dgermination of the wheat crops. For improvement of utilization efficiency on potassium fertilizer, the invention has important practical value and theoretical significance.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and in particular relates to a nucleic acid sequence of a novel wheat potassium ion channel gene and its application. Background technique [0002] In recent years, the multiple cropping index of my country's cultivated land has been continuously increasing, and the input of nitrogen and phosphorus is increasing day by day. However, due to the lack of potassium resources, the overall input of potassium fertilizer is insufficient, and the scope of potassium-deficient soil in the country is gradually expanding. Some production areas also have low potash utilization efficiency, resulting in a large waste of potash resources. In addition, my country's potash resources are extremely scarce, and potash fertilizers mainly rely on imports, which are expensive and cannot meet the needs of large-scale planting. Therefore, improving the efficiency of potassium fertilizer use has important pr...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 郑有良王峰王际睿魏育明蒲至恩江千涛
Owner SICHUAN AGRI UNIV
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