Sulfamethazine antigen, preparation method and application thereof

A technology of sulfamethazine and antigen, applied in the field of sulfamethazine antigen and its preparation and application, can solve problems such as affecting the recognition of animal body, achieve improved probability, high purity and yield, and cross-reaction rate low effect

Inactive Publication Date: 2011-07-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the spacer arm containing four carbon atoms can rotate freely in space, so that the linked sulfamethazine molecule may be covered by the carrier protein, affecting the recognition of the animal body

Method used

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  • Sulfamethazine antigen, preparation method and application thereof
  • Sulfamethazine antigen, preparation method and application thereof
  • Sulfamethazine antigen, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, preparation sulfamethazine hapten

[0023] 1) Dissolve 1.0g of sulfamethazine in 80mL of acetone, add 10mL of anhydrous pyridine, and mix until it is completely dissolved;

[0024] 2) Dissolve 2.0g of maleic anhydride in 20mL of anhydrous acetone, and the mixed solution obtained in 1), and reflux in a water bath at 60°C for 2 hours, the color of the solution gradually turns red, until the end of the reaction, the color of the reaction solution turns black;

[0025] 3) Evaporate acetone and pyridine to dryness to obtain a black oily substance, add 100mL 5% (mass percentage) sodium carbonate solution, and wash twice with 20mL dichloromethane;

[0026] 4) Adjust the pH to neutral, add 50 mL of ethyl acetate for extraction, discard the aqueous phase, and dry under reduced pressure to obtain 825 mg of the sulfamethazine hapten with a yield of 82.5%.

[0027] 2. Structural identification of sulfamethazine hapten

[0028] Gained sulfamethazine hapten, determine...

Embodiment 2

[0033] Embodiment 2, preparation sulfamethazine antigen

[0034] 1) Preparation of sulfamethazine antigen

[0035] Weigh 10 mg of the sulfamethazine hapten of Example 1 and dissolve it in 5 mL of N, N-dimethylformamide (DMF), ultrasonically dissolve it completely, and cool with cold ethanol; then add 10 mg of N, N '-Dicyclohexylcarbodiimide (DCC) and 5mg N-hydroxysuccinimide (NHS), react overnight at room temperature; finally add bovine serum albumin or ovalbumin carbonate solution dropwise (50mg bovine serum albumin or ovalbumin dissolved in 5 mL of 0.01M carbonic acid solution), and continued to stir for 4 hours; then put the reaction solution into a dialysis bag, and dialyzed with normal saline solution at 4°C for 48 hours, changing the water 6 times. The dialysate was passed through a 0.2 μm filter membrane under sterile conditions to obtain the sulfamethazine antigen (SM 2 -BSA) or coating antigen (SM 2 -OVA), aliquoted into ampoule bottles and stored at -20°C.

[003...

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Abstract

The invention discloses a sulfamethazine, a preparation method and an application thereof. The sulfamethazine antigen is an antigen obtained by coupling a sulfamethazine hapten with carrier albumen. The sulfamethazine hapten is a compound and the structural formula is shown as formula I. The sulfamethazine antigen immune animal can prepare the antibody with higher appetency and specificity. The sulfamethazine antigen can be used for detecting the residue of sulfonamide.

Description

technical field [0001] The invention relates to a sulfamethazine antigen, a preparation method and application thereof. Background technique [0002] Sulfamethazine (Sulfamethazine, SM 2 ), is a synthetic antibiotic with stable properties, broad antibacterial spectrum, low toxicity, easy oral absorption and low price, and plays an important role in the control and treatment of livestock and poultry diseases. One of the biggest drugs. However, due to its long action time and metabolism time in the body, it is easy to remain in the animal body, and it may accumulate in the human body if it is ingested through any route. Drug accumulation concentration exceeding a certain value is harmful to human body function, and long-term accumulation will lead to the generation of sulfa drug resistance, resulting in epidemic infection of drug-resistant bacteria, and has potential carcinogenicity. Therefore, the Codex Alimentarius Commission of the United Nations and many countries have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D239/69C07K14/765C07K14/77C07K1/113G01N33/53G01N33/531G01N33/566
Inventor 沈建忠肖希龙张素霞丁双阳江海洋王战辉张静
Owner CHINA AGRI UNIV
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