A kind of rapid propagation method of blue fruit tree
A fruit tree, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of long breeding period, large variation of breeding offspring, low seed germination rate, etc. Provenance and variation, the effect of maintaining excellent characters
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[0033] (1) Inoculation of explants
[0034] In the middle of each month from April to September, an inoculation test was carried out with tender stems with axillary buds and semi-lignified stems as explants, treated with 20% 84 disinfectant for 10-30 minutes, 75% alcohol for 10-30s+0.1% HgCl 2 Dip for 5-8 minutes or 0.1% HgCl 2 Soak and disinfect for 5-8 minutes; the medium is 1 / 2MS+6-BA1.0mg.L -1 +IBA0.1mg.L -1 + 30g.L sucrose -1 + agar 6g.L -1 . pH 6.0.
[0035] the result shows:
[0036] The bud germination rate of tender stems and semi-lignified stems can reach 81.8% to 100%. Among them, the explants inoculated in mid-June and mid-September had the highest success rate.
[0037] The disinfection effect of 20% 84 disinfectant on blue fruit tree explants is relatively poor, and the preservation rate of explants is 76.9%; 75% alcohol is not conducive to bud germination; 0.1% HgCl 2 The sterilization effect is relatively the best, and the explant preservation rate ca...
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