Method for rapidly propagating tupelos

A fruit tree, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long breeding period, low seed germination rate, and large variation of breeding offspring, and achieves maintaining excellent traits and maintaining neatness and consistency. , Conducive to the preservation of provenance and variation

Inactive Publication Date: 2009-10-28
HEBEI ACAD OF FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the existing method has many problems such as long breeding period, low seed germination rate, large variation of the offspring, and limitation of reproduction by natural conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] (1) Inoculation of explants

[0034] In the middle of each month from April to September, an inoculation test was carried out using tender stems with axillary buds and semi-lignified stems as explants, treated with 20% 84 disinfectant for 10-30 minutes, 75% alcohol for 10-30s+0.1% HgCl 2 Dip for 5-8 minutes or 0.1% HgCl 2 Soak and disinfect for 5-8 minutes; the medium is 1 / 2MS+6-BA1.0mg.L -1 +IBA0.1mg.L -1 + 30g.L sucrose -1 + agar 6g.L -1 . pH 6.0.

[0035] the result shows:

[0036] The bud germination rate of tender stems and semi-lignified stems can reach 81.8% to 100%. Among them, the explants inoculated in mid-June and mid-September had the highest success rate.

[0037] The disinfection effect of 20% 84 disinfectant on blue fruit tree explants is relatively poor, and the preservation rate of explants is 76.9%; 75% alcohol is not conducive to bud germination; 0.1% HgCl 2 The sterilization effect is relatively the best, and the explant preservation rate c...

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PUM

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Abstract

The invention discloses a method for rapidly propagating tupelos, which comprises the following steps of: a. explant inoculation; b. proliferation culture; c. root induction; and d. acclimatization and transplanting. The method facilitates the seed source retention and variation, maintains excellent traits of tupelos as well as the consistency of seedlings, has the advantages of rapid and mass superior cultivar propagation, and can produce mass tupelo seedlings in line within 6 months to 7 months.

Description

technical field [0001] The invention relates to tree seedling cultivation and afforestation techniques, in particular to a method for tissue culture and asexual propagation of blue fruit trees. Background technique [0002] Nyssa sinensis (Nyssa sinensis), also known as purple tree, is a tall deciduous tree belonging to the genus Nyssa sinensis. It is named for its dark blue skin when the drupe is ripe. Blue fruit tree likes light and acidic soil, and has strong adaptability. The trunk is straight, the branches and leaves are dense, the leaves are broad and gorgeous, and the ripe fruit is beautiful dark blue. It is an excellent tree species integrating ornamental and practical. The propagation method of this nursery stock is mainly seed propagation at present. Such as "Modern Agricultural Science and Technology", No. 24, 2008, Lishui City Blue Fruit Tree Wild Resources Investigation and Seedling Afforestation Technology, "Forestry Practical Technology" 2006, No. 4 Blue Fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王春荣曹福亮毕君
Owner HEBEI ACAD OF FORESTRY SCI
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