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Artificial fertilization and incubation method of sinocyclocheilus grahami

A technology of artificial hatching and golden thread barb, applied in climate change adaptation, fish farming, application, etc., can solve unseen problems and achieve the effects of simple operation, low cost and high hatching rate

Inactive Publication Date: 2009-10-14
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Through document retrieval, do not see and the same report of the present invention

Method used

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Embodiment Construction

[0009] From 2007 to 2009, the inventor hatched a total of more than 400,000 fish fry in the Dianchi Lake Goldfish by adopting the method of the invention at the Rare and Indigenous Fish Conservation Research Base of the Kunming Institute of Zoology, Chinese Academy of Sciences, with a hatching rate of 95%. The specific methods are as follows:

[0010] a. Dry fertilization: Maleic acid (DOM) Dioketone produced by Ningbo No. 2 Hormone Factory was used for sexually mature broodstock at the rate of 1mg per kg body weight and LH A 2 (LHRH-A 2 ) Perform intramuscular injection at 1 μg per kilogram of body weight, and implement artificially assisted delivery 24 hours after the injection; and then squeeze the eggs and sperm of sexually mature female and male Dianchi golden barbs from their abdomens into the vessel gently, lightly with chicken feathers. After stirring lightly for 30 seconds, add water, the amount of water should be enough to cover the sperm, and stir for 20 seconds to...

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Abstract

The invention relates to an artificial fertilization and incubation method of sinocyclocheilus grahami, which belongs to the technical field of fishery product culture. The method comprises the steps of dry fertilization and artificial incubation. Intramuscular injection is performed on sexually matured parent sinocyclocheilus grahami with DOM with the use level of 1 mg for per kilogram body weight and luteinizing hormone releasing hormone A2 with the use level of 1 mug for per kilogram body weight; 24 hours later, the mature germ cell and the mature sperm are slightly squeezed out from the abdomens of female sinocyclocheilus grahami and male sinocyclocheilus grahami respectively; the germ cell and the sperm are slightly stirred for 30 seconds in a vessel by chicken feather; clear water is added into the germ cell and the sperm and stirring is performed for 20 seconds; the germ cell and the sperm are cleaned for three times by clear water; the germ cell and the sperm are uniformly spilled onto a palm sheet which is cleaned and sterilized and put into 5 ppm mould solution to be dipped and sterilized for 15 munities, then the germ cell and the sperm are taken out and put into a 20 m or 50 incubating pond to be incubated; an oxygen pump is used for oxygenation to performing incubation under the condition of 18 to 20 DEG C water temperature and 7.5 to 8.0 pH, and after 150 hours, the incubation is accomplished. The method has the advantages that the cost is low, the operation is simple, the method can be put into industrial and intensive production, and the fertilization rate and the incubation rate are high.

Description

Technical field: [0001] The invention relates to an artificial insemination and hatching method of Dianchi golden barb, belonging to the technical field of fish product culture. Background technique: [0002] Dianchi golden thread carp (Sinocyclocheilus grahami Regan) is also known as Dianchi golden thread fish, pineapple fish, and small hole fish. It is distributed in the rivers, Rongdong and dark rivers where Dianchi Lake and its basin are located. Dianchi golden thread barb has delicious meat and is the first of the "Four Famous Fish" in Yunnan. It has extremely high development value and market prospects. In the past ten years, due to the severe pollution of Dianchi Lake, the water quality has become inferior to Class 5 water, and the habitat and reproduction environment of Dianchi Golden Barb has been severely damaged. It was listed as a national second-level protected animal, and was included in the "Rare Aquatic Animal Protection List of Yunnan Province" in the foll...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 杨君兴潘晓赋李再云陈小勇崔桂华
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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