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Preparation method of high-purity monomer flavone and general flavone contained in capsella bursa-pastoris and application of general flavone

A technology of total flavonoids and high purity, which is applied in the field of medicine, can solve the problems of no chemical components in shepherd's purse, no relevant literature and patents of shepherd's purse, and few shepherd's purse, and achieve the effect of low cost, increased content, and simple extraction method

Inactive Publication Date: 2009-09-23
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research on shepherd's purse is less, there is no research on the chemical components in shepherd's purse, and there are no relevant literature and patents on the use of total flavonoids in shepherd's purse for cardiovascular diseases

Method used

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  • Preparation method of high-purity monomer flavone and general flavone contained in capsella bursa-pastoris and application of general flavone
  • Preparation method of high-purity monomer flavone and general flavone contained in capsella bursa-pastoris and application of general flavone
  • Preparation method of high-purity monomer flavone and general flavone contained in capsella bursa-pastoris and application of general flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the preparation of shepherd's purse total flavonoids

[0023] Dried the harvested shepherd's purse medicinal material and cut it into sections. Add 10 times the crude drug amount of 90% ethanol to the obtained shepherd's purse crude drug and reflux extract it for 3 times, each extraction time is 1.5h, collect the extract, filter, and concentrate the obtained filtrate Dried to obtain shepherd's purse extract extract, the extract obtained is loaded on HPD-600 type macroporous resin with 0.5g / ml, and the flow rate of sample loading is 3BV / h, and adopting column diameter height ratio is 1: 5, uses 70% concentrated ethanol solution for elution. The eluate is filtered, the filtrates are combined, concentrated, dried and pulverized to obtain the total flavonoids of shepherd's purse. Shepherd's purse total flavonoids of the present invention, it is yellow-brown powder, with 2 "-O-α-L-arabinoisoorientin as reference substance, adopt spectrophotometry, measure absorb...

Embodiment 2

[0024] Embodiment 2, the preparation of shepherd's purse total flavonoids

[0025] Dried the harvested shepherd's purse medicinal material and cut it into sections, adding the obtained shepherd's purse crude drug to 8 times the crude drug amount of 60% methanol for cold extraction for 12 hours, collecting the filtrate, filtering, concentrating and drying the obtained filtrate, and obtaining the extract of shepherd's purse, The obtained extract was loaded on HPD-600 type macroporous resin at 0.4g / ml, the sample loading flow rate was 2BV / h, the diameter-to-height ratio of the column was 1:5, and 30% concentration ethanol solution was used for elution. The eluate is filtered, the filtrates are combined, concentrated, dried and pulverized to obtain the total flavonoids of shepherd's purse. Shepherd's purse total flavonoids of the present invention, it is yellow-brown powder, with 2 "-O-α-L-arabinoisoorientin as reference substance, adopt spectrophotometry, measure absorbance at 27...

Embodiment 3

[0026] Embodiment 3, the preparation of shepherd's purse total flavonoids

[0027] Dried the harvested shepherd's purse medicinal material and cut it into sections, adding 5 times the crude drug amount of 50% methanol to the obtained shepherd's purse and ultrasonically extracting it for 0.5h, collecting the filtrate, filtering, concentrating and drying the obtained filtrate, and obtaining the extract of shepherd's purse, The obtained extract was loaded on HPD-600 type macroporous resin at 0.3 g / ml, the sample loading flow rate was 1 BV / h, the diameter-to-height ratio of the column was 1:7, and 50% ethanol solution was used for elution. The eluate is filtered, the filtrates are combined, concentrated, dried and pulverized to obtain the total flavonoids of shepherd's purse. Shepherd's purse total flavonoids of the present invention, it is yellow-brown powder, with 2 "-O-α-L-arabinoisoorientin as reference substance, adopt spectrophotometry, measure absorbance at 270nm, calculate...

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Abstract

The invention relates to a preparation method of a general flavone and several high-purity monomer flavones contained in capsella bursa-pastoris and the application of the general flavone on angiocardiopathy, the general flavone and the several high-purity monomer flavones are flavonoid compounds from the capsella bursa-pastoris. The preparation method of the general flavone comprises the following steps: medicinal material extraction and general flavone preparation, i.e. dry herbs of the capsella bursa-pastoris are extracted by alcohol reflux, ultrasound or cold soaking and then put onto a column by using macroporous resins and eluted by 30-90 percent of the alcohol, and alcohol eluate is collected and then concentrated and dried to obtain the general flavone with the content of more than 60 percent. The general flavone prepared by the method can be used for preparing drugs for treating the angiocardiopathy. A preparation method of the high-purity monomer flavones comprises the medicinal material extraction, separation and purification. The preparation method is simple, practical, fast and convenient, has low cost and is suitable for industrialized production.

Description

technical field [0001] The invention relates to the field of medicine, and relates to a preparation method of total flavonoids derived from shepherd's purse medicinal materials and an application thereof in preparing medicines for treating cardiovascular diseases. Background technique [0002] According to the report of the World Health Organization in October 2008: Cardiovascular disease, infectious disease and cancer are the three main causes of human death at present, among which the number of people who die from cardiovascular disease is the largest, accounting for 29% of the total death toll in the world. According to the analysis of the World Heart Federation, the global cardiovascular disease mortality rate will increase by 50% in 2020, and the number of cardiovascular disease deaths will reach 25 million, of which 19 million will occur in developing countries. At present, cardiovascular disease is also the "number one killer" of Chinese people's health. Nearly 3 mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/31A61K31/7048A61P9/00
Inventor 刘晓秋宋宁兰洲王荣荣
Owner SHENYANG PHARMA UNIVERSITY
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