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Paenibacillus polymyxa for preventing and treating plant fungal diseases and production method thereof

A polymyxa-like spore and plant disease technology, applied in the direction of microorganism-based methods, botany equipment and methods, plant growth regulators, etc., can solve problems such as polluted water bodies and the environment, soil deterioration, and difficult treatment, and achieve production costs The effect of low and high-efficiency control

Inactive Publication Date: 2009-09-02
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent (authorized announcement number: CN 1186441C) discloses that Paenibacillus polymyxa can be used as a component of microbial fertilizer inoculum to decompose straw, which effectively solves the problem of excessive agricultural waste in agricultural production, polluting water bodies and the environment, and processing difficulties. On the one hand, the excessive use of chemical fertilizers and pesticides leads to the contradiction of soil deterioration
There is no literature report on the method of producing Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater as a medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: preparation of Paenibacillus polymyxa biopesticide

[0027] Preparation of seed medium: Weigh 200g of potatoes, chop them, boil in 1L of tap water for 30 minutes, filter to remove residue, add 20g of glucose, and dilute to 1L to obtain potato medium. Divide into five 1L Erlenmeyer flasks and sterilize at 121°C for 20 minutes.

[0028] Fermentation medium preparation: add 1 L of monosodium glutamate wastewater flocculated to remove bacterial protein into 9 L of tap water to dilute, and adjust the pH to 6.0 with sodium hydroxide.

[0029] Liquid seed preparation: inoculate Paenibacillus polymyxa into potato liquid medium, shake the flask at 200 r / min at 30° C., shake the flask at a constant temperature for 24 hours, and obtain liquid seeds.

[0030] Fermentation product: Fill the above fermentation medium into a 10L fermenter, sterilize at 121°C for 15 minutes, inoculate liquid seeds with an inoculum of 5%, and ferment for 2 to 3 minutes at a constant tempe...

Embodiment 2

[0031] Embodiment 2: Antagonism to various pathogenic fungi

[0032] Bacterial strain inoculates the pathogenic fungus that diameter is 1cm at the PDA solid medium plate center place, inoculates (dot connects) the good Paenibacillus polymyxa that activates at 2cm from the edge of pathogenic fungal colony, observes the size of inhibition zone on the 6th day, finds that Pathogenic fungi Botrytis cinerea, cucumber scab pathogens (Cladosporium sp.) and Trichoderma sp. all have significant antagonistic effects.

Embodiment 3

[0033] Embodiment 3: to cucumber scab control effect

[0034] When the cucumber seedlings in the greenhouse pot grow to four true leaves, use 10 7 / ml Paenibacillus polymyxa bacterium liquid spray treatment leaves once, after interval 1 day, inoculate scab pathogen, then after interval 1 day, spray 10 7 Paenibacillus polymyxa bacterium liquid once, replace the cucumber seedling of Paenibacillus polymyxa bacterium liquid spray with clear water as contrast, investigate the state of an illness of cucumber seedling continuously, find that Paenibacillus polymyxa treatment has obvious effect on cucumber scab Control effect.

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Abstract

The invention relates to paenibacillus polymyxa for preventing and treating plant fungal diseases and a production method thereof, pertaining to the technical field of biological pesticide. A bacterial strain adopted in the invention is a paenibacillus polymyxa EBL-06 strain separated from the leaf surface of wheat and saved in China General Microbiological Culture Collection Center (CGMCC) with a preservation code of CGMCC No.2377. The strain can prevent and treat various fungal diseases of plants to increase crop output. The invention utilizes waste monosodium glutamate water to ferment paenibacillus polymyxa for preparing a biocontrol microbial agent. The biocontrol agent of paenibacillus polymyxa can effectively control various fungal diseases of plants, has low production cost and simple operation, and can also effectively utilize waste monosodium glutamate water to reduce environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides. technical background [0002] According to the pollutants contained in the waste water in the MSG production process, it can be divided into three categories: one is high-concentration and high-acidity organic waste water, that is, off-mating liquid; the other is other medium and high-concentration organic waste water; the third is cooling water that does not need to be treated and is directly discharged outside. The off-mating tailing liquid is the remaining "waste liquid" after extracting glutamic acid by ion exchange method. It not only contains rich organic matter, but also contains a small amount of inorganic salts such as N, P, K and other trace elements, which can be used for microbial growth. Provides nutrition. Other medium and high concentration organic wastewater includes: starch wastewater, sugar wastewater, fermentation and irrigation wastewater, refined wastewater, et...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C02F3/34C12R1/12C02F103/32
Inventor 白志辉谷立坤王微张建云张洪勋庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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