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Sarracenia asepsis sowing and culture quick reproduction method

A technology of aseptic sowing and tissue culture rapid propagation, which is applied in the fields of botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems restricting the industrial production of Sarracenia sarracenia, and achieve robust and uniform tissue culture seedlings, transplanted and survived The effect of high rate and fast reproduction speed

Inactive Publication Date: 2009-09-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To a certain extent, it restricts the factory production of Sarracenia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This Sarracenia aseptic seeding and tissue culture rapid propagation method is carried out as follows:

[0022] 1), seed collection: adopt Sarracenia as parent, select the mother plant that grows robustly to carry out self-pollination and hybridization of Sarracenia during flowering, and use as explant when the capsule matures 120 to 150 days after pollination;

[0023] 2), aseptic sowing: take out the seeds from the capsule, soak the seeds in a 20% sulfuric acid solution by volume for 5 to 10 hours, then disinfect the surface with 75% alcohol by volume for 30 to 45 seconds, rinse with sterile water 3 times, and then use Soak in sodium hypochlorite solution and shake for 20-30 minutes to sterilize, rinse with sterile water for 3-5 times; inoculate the sterilized seeds on the seed germination medium under aseptic conditions, and cultivate them under culture conditions for 30-45 days. Seeds germinate into seedlings; culture temperature is 25±2°C, dark culture for 30-45 da...

Embodiment 2

[0030] In this example, the seed germination medium is: 1 / 6MS; cluster bud proliferation medium: 1 / 3MS+BA 1.0mg / L+NAA0.1mg / L; strong seedling medium: 1 / 4MS+BA 0.5mg / L +NAA0.05mg / L; rooting medium: 1 / 5MS+NAA 0.5mg / L.

[0031] All the other steps and conditions are the same as in Example 1.

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PUM

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Abstract

The invention belongs to the technical field of plant reproduction and mainly provides a sarracenia asepsis sowing and culture quick reproduction method which comprises the following steps: the collection of seeds, the asepsis sowing, the enrichment culture of cespitose shoots, the culture of sound seedling, the culture of rootage, and the transplanting. The basic culture medium is as follows: selecting 1 / 6MS-1 / 3MS culture medium, and adding 20-30g / L of cane sugar or white sugar and 7-9 g / L of agaragar and having the pH of 5.6-5.8. The invention has quick reproduction speed, uniform production sturdiness and high transplanting survival rate, and is suitable to the massive production because a large number of better culture seedlings are formed in a short period.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for aseptic sowing and tissue culture rapid propagation of Sarracenia. Background technique [0002] Sarracenia (Sarracenia), also known as cobra flower, belongs to the genus Sarracenia, which is native to Western Europe, North America and Mexico. Sarracenia is a perennial herb with rhizomes, creeping, and fibrous roots. The leaves are evergreen, rough, cylindrical, bottle-shaped with a top cover, and the base forms a rosette-shaped leaf cluster. Each bottle-shaped leaf is an insect trap. The bottle secretes mucus and digestive juices, which will stick to the bottle. The insects inside are digested and absorbed. Sarracenia scapes are upright, flowers are solitary, drooping, purple or green-purple, open from April to May, the flower stems are pulled out from the base of the leaves, the flowers are large and very characteristic, the flower ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 徐刚陈剑平汪一婷吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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