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Preparation method of liposome

A technology of liposomes and spheres, which is applied in the direction of liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., which can solve the problems of inability to increase the thickness of lipid film, low feasibility, limitations, etc. problems, to achieve the effects of low manufacturing cost, wide applicability, and simple equipment structure

Inactive Publication Date: 2009-08-19
中国人民解放军济南军区第四〇一医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The thickness of the lipid film cannot be increased, only the area of ​​the inner wall of the container can be increased, and the capacity of the container needs to be increased. Obviously, increasing the capacity of the container will bring a series of requirements to the manufacturing process, manufacturing requirements, and placement space of the container. According to the problem solved, the realization of industrialization will also be subject to many restrictions, and the feasibility is not high

Method used

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  • Preparation method of liposome
  • Preparation method of liposome

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preparation example Construction

[0035] The preparation steps of liposome of the present invention are:

[0036] Step 1: Put the organic solvent into a container that meets the pharmaceutical requirements,

[0037] Step 2: adding film-forming material and fat-soluble drug to the container,

[0038] Step 3: stirring or heating under reflux to fully dissolve the contents of the container,

[0039] Step 4: Put the microbeads into the above container,

[0040] Step 5: Rotate, heat the container in a water bath and evacuate, evaporate and remove the organic solvent,

[0041] A layer of lipid film is formed on the inner wall of the container and the surface of the microbeads,

[0042] Step 6: further drying the formed lipid film,

[0043] Step 7: Add the water phase or the water phase containing the drug, so that the lipid film forms liposomes from the inner wall of the container and

[0044] The surface of the microbeads falls off.

[0045] On the basis of the above scheme, said step 2 can also be: adding fi...

Embodiment 1

[0054] Embodiment 1 - the preparation of dexamethasone acetate liposome

[0055]Take 0.65g of egg yolk phospholipids, 0.125g of cholesterol, 20mg of dexamethasone acetate, and 30mg of vitamin E in a 250ml round bottom flask, dissolve them in 100ml of ethanol, add glass beads with a diameter of 3mm, vacuum at 11KPa, temperature at 46°C, and rotate at 30rpm Evaporate for 30 minutes to remove the organic solvent, and a layer of lipid film is formed on the surface of the microbeads. Put the flask into a vacuum dryer or blow hot air into the flask to further dry the lipid film, and add pH7.38 phosphate containing sodium sulfite to the flask Buffer solution, rotate or shake the flask, the lipid dry film will soon form a milky white liposome liquid, and there is no sediment and suspended matter in the emulsion.

[0056] Take the liposome liquid prepared above, add it into molten polyethylene glycol 6000 according to the weight ratio of 1:1.5, mix evenly, and then the dropping pill ca...

Embodiment 2

[0057] Embodiment 2 - the preparation of vitamin E liposome

[0058] Take 0.65g of egg yolk phospholipids, 0.125g of cholesterol, and 58mg of hydroquinone in a 250ml round bottom flask, dissolve them in 100ml of dichloromethane, add glass beads with a diameter of 2mm, vacuum at 12KPa, temperature at 35°C, and 50rpm rotary evaporation for 32 minutes Remove the organic solvent and form a layer of lipid film on the surface of the microbeads. Put the flask into a vacuum dryer or blow hot air into the flask to further dry the lipid film. Add pH7.42 boric acid containing sodium bisulfite to the flask Salt buffer solution, rotating or shaking the flask, the lipid dry film will soon form a milky white liposome liquid, and there is no sediment and suspended matter in the emulsion. The prepared vitamin E liposome liquid can be used as skin care liquid or cosmetics.

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Abstract

The invention provides a method for preparing a liposome, and relates to preparation of a drug carrier and a cosmetic carrier. The preparation comprises: step 1, adding an organic solvent in a container meeting medical requirements; step 2, adding a film forming material and a fat soluble medicine in the container; step3, fully dissolving the materials in the container by stirring or reflux heating; step 4, adding microspheres in the container; step 5, rotating and waterbath heating the container, vacuum pumping the container, and removing the organic solvent by evaporation to form a fat layer on the inner wall of the container and the surfaces of the microspheres; step 6, further drying the formed fat layer; and step 7, adding a water phase or medicine-containing water phase, so that the fat layer forms the liposome and falls off the inner wall of the container and the surfaces of the microspheres. The method has the advantages of simple and effective preparation process, low cost, obviously higher attachment area of the fat layer compared with the prior art and the realization of the industrialization in the preparation of the liposome, and the size of the microspheres can be changed so as to generate the various liposome, so that the method has wide adaptability.

Description

technical field [0001] The invention relates to a preparation method of a drug carrier and a cosmetic carrier in the field of medicine and cosmetics, in particular to a preparation method of a liposome. Background technique [0002] Liposome is an excellent drug carrier, which can be prepared by thin film dispersion method, but it is difficult to realize industrialization by thin film dispersion method. [0003] In the existing film dispersion method, the film-forming materials such as phospholipids and cholesterol and the fat-soluble drugs are put into a container equipped with an organic solvent, and the film-forming materials such as phospholipids and cholesterol and the fat-soluble drugs are dissolved in the organic solvent. It can be chloroform, and the container is generally a round bottom flask, then rotate and heat the container, remove the organic solvent by heating and evaporating, and form a layer of lipid film (lipid dry film) on the inner wall of the container, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K45/06A61K47/24A61K31/573A61K31/355A61K31/203
Inventor 马守栋李明春梁东升俞发
Owner 中国人民解放军济南军区第四〇一医院
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