Ultra-low temperature cryopreservation method and cryopreservation device of large yellow croaker sperm
A cryopreservation method and technology of large yellow croaker, which can be applied to household refrigeration equipment, human or animal body preservation, lighting and heating equipment, etc., and can solve the problems of poor applicability of cryopreservation, poor visibility of microscopic tubes, and poor quality of sperm preservation and other problems, to achieve good antifreeze effect, reasonable mixing time and low cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Such as figure 1 The cryopreservation device for the cryopreservation of large yellow croaker sperm includes a foam box 1 with a cover 2, liquid nitrogen 3 is built in the foam box 1, the inner length of the foam box 1 is 16 cm, the width is 12 cm, and the depth is 18 cm , the wall thickness is 2.5 centimeters, on the two long walls 12 of the foam box 1, there are grooves 4 downward from the top 11 of the foam box 1, and the depth of the groove 4 downward from the top 11 of the foam box 1 0.5 cm, the straw 5 is placed in the groove 4, and the distance between the liquid level 31 of the liquid nitrogen 3 and the groove 4 is 2.5 to 3.5 cm.
Embodiment 2
[0026] The method for ultra-low temperature freezing of large yellow croaker sperm, the steps are as follows:
[0027] 1) Antifreeze preparation: Mix the diluent and antifreeze evenly according to the volume ratio of 86.7:13.3, prepare the antifreeze and store it at 4°C for later use. The components of the diluent are as follows:
[0028] NaCl: 7.25 grams KCl: 0.38 grams CaCl 2 : 0.18g
[0029] NaHCO 3 : 1.00 g MgSO 4 ·7H 2 O: 0.23 g NaH 2 PO 4 2H 2 O: 0.41g
[0030] Glucose: 1.00 g, dissolved in distilled water and fixed to 1000 ml; antifreeze agent is DMSO.
[0031] 2) Sperm collection: Select male large yellow croakers with mature gonads, dry the body surface around the genital opening, lightly press the belly of the fish to obtain semen, put it in a petri dish or a small beaker, and put it on an ice bath. It is more ideal to use the sperm with stronger vitality and longer activity time to be used for cryopreservation.
[0032] 3) Preparation of the mixture of ant...
Embodiment 3
[0036]The activation rate of the frozen sperm of the present invention is compared with the motility time, lifespan and relative fertilization rate of fresh sperm after activation, and the data shown in Table 1 are obtained, which shows that the activation rate of the frozen sperm of the present invention is higher. The quality is better, and the relative fertilization rate is also higher.
[0037] Table 1 Comparison table of activation rate, movement time after activation, lifespan and relative fertilization rate between frozen sperm and fresh sperm:
[0038]
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com