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Predominating Population for removing ethanethiol and introduction domestication method thereof

A technology of ethanethiol and dominant bacteria, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of few researches on special-effect dominant bacteria, achieve low cost, easy method, The effect of low running cost

Inactive Publication Date: 2009-08-05
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the specific dominant bacteria, especially for the seriously harmful ethanethiol degrading bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Introduction domestication method of the present invention, comprises the following steps:

[0035] a. Select a city riverside to take mud samples to collect mixed bacteria, put them into sterile paper bags for the next step.

[0036] b. Using beef extract peptone medium added with ethanethiol as the acclimatization medium, prepare flat solid acclimatization medium and slant solid acclimation medium. Wherein the acclimation medium comprises the following components:

[0037] Beef Extract 1.5g

[0038] Peptone 8g

[0039] NaCl 5g

[0040] Ethanthiol 1.3g

[0041] Distilled water 85.2g

[0042] pH 7.0~7.2

[0043] The liquid culture medium prepared according to the above-mentioned drugs and dosage should be heated in a microwave oven to mix the drugs evenly. Heat and sterilize at 1.1kPa and cool down for use.

[0044] c. In the original strain mixture, add nutrients every day, and cultivate at a constant temperature in an environment of 25°C. The composition and ...

Embodiment 2

[0055] The introduction and acclimation method of this example differs from Example 1 in that the mixed bacteria are collected in sulfide-containing industrial sludge (such as tannery wastewater).

Embodiment 3

[0057] The introduction and domestication method of the present embodiment is different from Example 1 in that the nutritional ingredients include the following compositions:

[0058] Glucose 5.8g

[0059] Urea 0.25g

[0060] Dipotassium hydrogen phosphate 0.074g

[0061] Magnesium sulfate 1.59g

[0062] Calcium chloride 1.91g

[0063] Ferrous sulfate 0.06g

[0064] In Example 3, the introduction and acclimatization method of the present embodiment differs from Example 1 in that the liquid acclimatization medium includes the following components:

[0065] Beef Extract 1.6g

[0066] Peptone 8g

[0067] NaCl 4.5g

[0068] Ethanthiol 0.25g

[0069] Distilled water 85.65g

[0070] pH 7.0~7.2

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PUM

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Abstract

The invention relates to preponderant strains for removing ethanethiol and an introduction domestication method thereof. Quantitative mixing strains from different resources of mud samples in live mud, industrial mud, and the like, which contain sulfur or sulphide, are obtained. The mixing strains are domesticated and cultured in a culture medium with ethanethiol, after a definite time, bacteria in the culture medium have the characteristic of definite ethanethiol removal. Bacterial strains are separated many times by using a continuous dilution separation method and a plate streaking method so as to obtain preponderant strains with ethanethiol removal. The strain is CTB138-1 AY345539.1 through DNA determination and the scanning and observation of an electron microscope, and the bacteria are in a short rod shape. The ethanethiol removal rate can reach more than 85 percent through determination result indication.

Description

technical field [0001] The invention relates to a deodorizing (odor) bacterium and a domestication method thereof, in particular to a domestication method of a dominant bacterium for removing ethanethiol. Background technique [0002] Ethyl mercaptan is the main component of sulfur-based malodorous gases, because it is often combined with H 2 Mixed emissions of sulfur-based odorous gases such as S and methyl mercaptan, and the content is much lower than that of H 2 S, therefore often neglected by people. However, it has a wide range of odor sources and high toxicity. Short-term inhalation will cause people to lose their mind, anorexia or vomiting; long-term inhalation will damage people's central nervous system and blood circulation system. The olfactory threshold of ethanethiol is low, and the content of ethanethiol in the air can produce odor when the content reaches one part per billion, and it is easy to be absorbed by clothes, skin, cornea and nasal mucosa, etc., and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B01D53/84B01D53/52C12R1/01A62D101/28
CPCY02A50/20
Inventor 郭斌任爱玲
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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