Method for extracting, separating and purifying genistein from pigeon pea rhizome

Abstract

The invention relates to a method for extracting, separating and purifying an active component of Chinese herbal medicine, namely genistein from roots and stems of pigeon pea, and aims at providing a method for easily, safely, economically and effectively extracting, separating and purifying the high-purity genistein from roots and stems of the pigeon pea. The adopted technical proposal comprises that: fresh or dry roots and stems of the pigeon pea are used as raw materials, and by adopting the homogenate induction technique, the enzyme and acid induction technique, the solvent extraction technique, the ultrasonic oscillation flocculation technique, the negative pressure cavitation suspension solid extraction technique, the macroporous absorbent resin enriching technique, the positive phase silica gel medium-pressure column chromatography separation technique and the low-temperature crystallization and re-crystallization technique, the refined genistein is obtained, and the purity of the genistein reaches more than 95 percent. The method has the advantages of abundant and easily obtained raw materials, simple and convenient process, high yield and purity of the obtained genistein, suitability for industrialized production and high added value of products.

Description

technical field [0001] The present invention relates to a method for extracting, isolating and purifying active ingredients from plants, in particular extracting, isolating and purifying genistein from fresh or dried rhizomes of pigeonpea. Background technique [0002] Genistein is an isoflavone compound widely found in legumes and is an estrogen-like compound. In vivo metabolism, it can combine with endogenous estrogen receptors to exert estrogen-like effects, and is called phytoestrogens. In medicine, it is mainly used to treat gynecological diseases and senile osteoporosis caused by lack of estrogen. In today's world, due to the aging population, the incidence of senile diseases such as osteoporosis is on the rise, and estrogen drugs have a good market prospect as the main drug for osteoporosis treatment. Isoflavone estrogens have estrogenic activity only in the form of glycosides. Genistein is the glycogen of isoflavones, which is easy to absorb and can directly play a...

AI Technical Summary

Problems solved by technology

However, there are few literature reports on the purification process, and the existing methods for extracting and purifying genistein are mainly traditional alcohol extraction and column chromatography purification.

The traditional purification process is long and the loss of the target compound is large. Multiple silica gel column chromatography is used. The operation steps are numerous and the eluent is mainly toxic and expensive reagents such as chloroform, acetone, and ethyl acetate.

In the Chinese patent "Extraction method of genistein" (publication number CN1465571), traditional alcohol extraction, concentration, water precipitation, reflux, suction filtration, heating reflux hydrolysis and other methods are used to obtain genistein. This method is not fine enough, and the amount of organic solvent used Large, high product impurity content, low yield

[0007] In summary, in the process of using plant resources to isolate pure genistein, the main influencing factors are improper selection of raw materials, backward extraction technology, widespread loss of genistein in the process of raw material treatment and separation, and long purification process. cost increase

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