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Bacterial vaginopathy combined detection reagent

A technology for combined detection and vaginosis, applied in the direction of microbiological measurement/testing, microbiology, biochemical equipment and methods, etc., can solve the problems of unscientific, non-objective and inaccurate vaginosis, and achieve low cost and comprehensive and reliable measurement , Simple and fast operation

Inactive Publication Date: 2009-07-22
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Public documents such as application number is 200610056827.X Chinese invention application publication "bacterial vaginosis joint assay kit" (publication number: CN1850985A), adopt hydrogen peroxide reagent, leukocyte esterase reagent and sialidase in this application The combination of reagents overcomes the deficiencies of unobjective, unscientific and inaccurate diagnosis of vaginosis by traditional single means

Method used

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Examples

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Embodiment

[0017] Embodiment: The combined detection reagent for bacterial vaginosis in this embodiment is placed in a kit, specifically including hydrogen peroxide reaction buffer, sialidase reaction buffer, leukocyte esterase reaction buffer, pH Reaction pads, diluents and indicators.

[0018] Hydrogen oxidation reaction cushion solution includes the following components and proportions: 0.1-0.5mM ferrous chloride, 0.1-0.5mM starch, 10-50mg / L 2-hydroxy-3,5-dichlorobenzenesulfonic acid Sodium salt, 300-500U enzyme activity / L horseradish peroxidase, 2-mercaptoethanol with a volume percentage of 0.01%, the buffer system is 0.1M PB solution, and the pH of the PB solution is 4-5;

[0019] The sialidase reaction cushion solution includes the following components and proportions: 0.1~1.0mM 5'-bromo-2'-chloro-indole-3'-oxysialoside or 5-bromo-4-chloro- 3-indolyl-α-D-N-acetylneuraminic acid or thymolphthalein acetylneuraminic acid, 0.1~1mg / ml 3-(N-p-toluenesulfonyl-L-alanyloxy)-indole Indole,...

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Abstract

The invention discloses a bacterial vaginosis joint detecting reagent, which comprises a hydrogen peroxide reaction pad foam fluid, a neuraminidase reaction pad foam fluid, a leukocyte esterase reaction pad foam fluid and a pH reaction pad foam fluid. Compared with the prior art, the bacterial vaginosis joint detecting reagent has the advantages of simultaneous detection of multiple data sets, respective reflection of microecological environment, pathogenic bacteria and secretions situations, more comprehensive and reliable detection, simple and quick operation, no need of instrument and equipment, and lower cost.

Description

technical field [0001] The invention relates to a detection reagent for in vitro diagnosis, in particular to a combined detection reagent for bacterial vaginosis. The detection reagent can evaluate vaginal micro-ecological environment, cleanliness and pathogenicity of bacterial vaginosis. Background technique [0002] Bacterial vaginosis (Bactarial Vaginosis, referred to as BV) is the most common vaginal infection in women of childbearing age. According to domestic reports, the prevalence of BV is 18.9% among healthy women and 43.3% among patients with abnormal vaginal discharge in gynecology clinics. %, 36.7% in sexually promiscuous people, and 12.5% ​​in pregnant women. It is due to the mixed bacteria composed of Gardnerella vaginalis, anaerobic bacteria, and Mycoplasma hominis from the normal vaginal flora (including Lactobacillus) The group is replaced, causing the disease caused by the imbalance of the flora. Although it does not belong to the STDs monitored in China, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/04C12R1/01
Inventor 陈文武
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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