Preparation of rare earth luminous nanoparticle based on pyridine dicarboxylic acid

Abstract

The invention relates to a preparation method of rare-earth luminescent nanometer particles based on pyridine dicarboxylic acid. The rare-earth luminescent nanometer particle comprises SiO2 coating luminescent rare-earth composition; the rare-earth composition is produced by ligand pyridine dicarboxylic acid combined with rare-earth ions; and SiO2 nanometer particles with active functional group on the surface are formed by the polymerization reaction of the rare-earth composition in the microemulsion and the silanization. The prepared rare-earth luminescent nanometer particle has high luminous intensity and good water solubility, and can be used for a variety of time-resolved fluorescence analysis and imaging. Compared with the homogeneous rare-earth nanometer particle preparation method, the preparation method has the advantages of low preparation cost and simple method, and can be widely applied.

Description

technical field [0001] The invention relates to a preparation method of rare earth luminescent nanoparticles based on dipicolinic acid, which is mainly used for fluorescence detection and tracing of biomolecules, and belongs to the technical field of preparation of biomolecular nanometer markers. Background technique [0002] Fluorescence detection and imaging technologies are widely used in many fields such as life sciences and medicine. However, various organic dye molecules used in traditional fluorescence analysis are susceptible to interference from various non-specific fluorescence (excitation light, scattered light and analyte background fluorescence ), and the dye molecules are prone to photobleaching and quenching effects, resulting in a decrease in fluorescence intensity. Nanomarkers are a new type of markers developed in recent years. Since nanoparticles are loaded with several fluorescent molecules through surface adsorption or embedding, the fluorescence emissio...

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Problems solved by technology

Since these methods use non-solution homogeneous preparation, the uniformity of the shape of the prepared particles, the controllability of the size, and the dispersion in the aqueous solution are not ideal.

[0007] Chinese Patent Publication No. CN 1493647A, 2004, Yuan Jingli, Tan Mingqian, Ye Zhiqiang, Wang Guilan, a functional nano-rare earth fluorescent particle and its preparation and application, discloses a particle preparation method for wrapping rare earth complexes, the method is to emit light Strong rare earth complexes as the center, using SiO 2 Wrapped into nanoparticles as markers, the prepared particles have good uniformity and strong fluorescence intensity, and can be used for time-resolved fluorescence analysis. However, the ligands of the rare earth complexes used have no commercial sources and require a multi-step organic reaction. Special synthesis, ligand preparation is complicated and not easy to obtain

Preparation of SiO by Solution Homogeneous Reaction 2 The key to encapsulated rare earth nanoparticles is to synthesize rare earth complexes with good water solubility, stability, and high luminous intensity. At present, there are few types of such rare earth complexes, and complex organic synthesis is required, which is expensive and difficult to obtain.

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