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Discovery method for protein post-translational modification

A technology of post-translational modification and method discovery, which is applied in material separation, material inspection products, instruments, etc., and can solve problems such as high computational complexity and high accuracy

Active Publication Date: 2009-07-08
INST OF COMPUTING TECH CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of high computational complexity and accuracy affected by the quality of experimental tandem spectra in the process of detecting protein post-translational modification using fragment ion spectrum information, thereby providing an efficient and accurate protein translation post-modification discovery method

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  • Discovery method for protein post-translational modification
  • Discovery method for protein post-translational modification
  • Discovery method for protein post-translational modification

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Embodiment Construction

[0068] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0069] Assuming that there is a protein sample, the protein sample is first enzymatically hydrolyzed by existing biochemical techniques to form a peptide mixture, and then separated by liquid chromatography (peptides with different physical and chemical properties have different chromatographic retention times), and then separated by mass spectrometry. The peptide mixture is separated according to the mass-to-charge ratio (mass-to-charge ratio for short), and the separated peptides are broken into fragment ions and detected to form a peptide fragment ion spectrum. The above operation process makes the experimental tandem mass spectrum generated by liquid chromatography-mass spectrometry have three kinds of information of peptide chromatographic retention time, peptide mass, and fragment ion spectrum. It should be noted that for high-precision m...

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Abstract

The invention provides a detecting method of post-translational protein modification. The method comprises the following steps: calculating spectrogram difference vectors among all spectrograms by means of peptide chromatogram retaining time and peptide quality in experimental tandem mass spectrum data of protein samples; establishing candidate modification mass intervals probably containing modification mass; estimating mixed distribution of the spectrogram difference vectors on each candidate modification mass interval, and calculating the standard deviation of each distribution inside the mixed distribution so as to determine distributions inside the candidate modification mass intervals caused by post-translational protein modification according to the standard deviation; calculating the average value of the distributions caused by post-translational protein modification; obtaining an accurate mass experimental value of the post-translational protein modification according to the mass component of the average value; and obtaining the influence of post-translational protein modification on peptide chromatogram retaining time according to the retaining time component of the average value. The detecting method has the advantages of high efficiency, accuracy and robustness.

Description

technical field [0001] The invention relates to protein post-translational modification in biogenetics, in particular to a method for discovering protein post-translational modification. Background technique [0002] It is well known that the genetic information of most organisms is stored in DNA. DNA generates messenger RNA through the process of transcription, and messenger RNA generates protein through the process of translation, thus realizing the transmission of genetic information from DNA to RNA and then to protein. This process is also known as the central dogma of life. In the process of protein translation from RNA, the chain molecules formed by connecting 20 kinds of amino acids in sequence with peptide bonds are called peptides, and the peptides whose molecular weight reaches a certain level are called proteins. After most proteins are translated, some functional groups (such as adding acetyl to the N-terminal of the protein) will be added to some amino acids in...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N33/68G06F19/00
Inventor 付岩贺思敏王海鹏孙瑞祥
Owner INST OF COMPUTING TECH CHINESE ACAD OF SCI
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