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Method for separating protein from bean curd yellow pulp water using flocculating reagent

A technology of flocculant and yellow slurry water, applied in the field of protein separation, can solve problems such as secondary pollution, and achieve the effect of improving quality, reducing usage and good flocculation effect

Active Publication Date: 2009-07-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive flocculant addition (0.15-0.3%) will also cause secondary pollution

Method used

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  • Method for separating protein from bean curd yellow pulp water using flocculating reagent
  • Method for separating protein from bean curd yellow pulp water using flocculating reagent
  • Method for separating protein from bean curd yellow pulp water using flocculating reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 1L of the aforementioned soybean wastewater, add 0.05mL, 0.15mL, 0.3mL, 0.4mL, 0.5mL, 0.75mL, 1.0mL and 2.5mL of 10% polyacrylamide solution respectively, and carry out the flocculation reaction in a 2L beaker, first stir rapidly Disperse the flocculant in 1min, and mix the flocculated suspension at 20°C, 100r / min mechanically for 5min, then stand at 4°C for 12h, centrifuge at 3000r / min for 5min, measure the turbidity of the supernatant, because the turbidity in the wastewater The turbidity value is closely related to its protein content, so the percentage of turbidity reduction can be used as an index to evaluate the protein recovery rate. The turbidity is measured by a turbidity meter of the model DRT100BHF, and distilled water is used as a blank. The experimental results are expressed in the percentage of turbidity reduction. (Stock solution turbidity-turbidity after flocculation) / turbidity of stock solution×100%, see Table 1 for details.

[0019] Table 1: Comp...

Embodiment 2

[0023] Take 1L of the aforementioned soybean wastewater, add 1.0, 3.0, 5.0, 8.0, 10, 20, 30, 40, 50mL of 10% polyaluminum chloride solution respectively, and carry out the flocculation reaction in a 2L beaker. After dispersing, the flocculated suspension was mechanically stirred and mixed at 20°C at 100r / min for 5min, then stood at 4°C for 12h, centrifuged at 3000r / min for 5min, and the turbidity of the supernatant was measured. See Table 2 for detailed data.

[0024] Table 2: Comparison of the effects of different concentrations of polyaluminum trichloride on flocculating proteins

[0025]

[0026] Within the experimental range, the flocculation effect of polyaluminum trichloride increases with the increase of its concentration. When the addition amount increases to 5mg / mL, the recovery rate of protein is close to 90%, but the addition amount is too high may cause secondary pollution.

Embodiment 3

[0028] Take 1L of the aforementioned soybean wastewater, add 1.0, 3.0, 5.0, 10, 20, 30, 40, 50mL of 10% polyferric sulfate solution respectively, and carry out the flocculation reaction in a 2L beaker. The final suspension was mixed at 20°C with 100r / min mechanical stirring for 5min, then stood at 4°C for 12h, centrifuged at 3000r / min for 5min, and the turbidity of the supernatant was measured. See Table 3 for detailed data.

[0029] Table 3: Comparison of the effects of different concentrations of polymeric ferric sulfate on flocculating proteins

[0030]

[0031] The optimal flocculation concentration of polyferric sulfate is 4mg / mL, and the recovery rate of protein will decrease if the concentration is too high or too low.

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Abstract

The invention provides a method for separating protein from yellow slurry of bean curd by using a flocculating agent, comprising the following steps: chitosan and one or more than two of the following mixtures (is) are added into the yellow slurry of bean curd as compound flocculating agent: polymerized aluminium trichloride, polymerized iron sulfate and sodium alginate; the total volume of the flocculating agent is 0.1-3mg / mL; the flocculating agent is first stirred and dispersed, then stirred and mixed mechanically for 5-10 min at 20 DEG C, placed still for 10-24h at the temperature between 0-4 DEG C, centrifugated and precipitated, finally, the protein is gained. In the invention, the combination of the chitosan and other flocculating agents is used as the compound flocculating agent, especially the natural giant molecule amphiprotic flocculating agent-chitosan composition is used, and compared with the traditional synthesized high molecule flocculating agent, the chitosan composition is more environmental friendly, the protein recycling rate is high, bigger molecule copolymer can be formed, the flocculating effect is good, the using amount is small, the cost is low, and the recycled protein product has good quality; in addition, the flocculating agent can be used in acid protein drinks or used as protein extender in the industries of baking, meet product, etc.

Description

(1) Technical field [0001] The invention relates to a method for separating protein from tofu yellow slurry water by using a flocculant. (2) Background technology [0002] The annual consumption of traditional soybean products in my country is close to 9 million tons. Based on the production of 8-10 tons of wastewater per ton of soybean processing, soybean product processing enterprises have nearly 10 million tons of high-concentration organic wastewater to be treated and discharged every year. The COD, BOD Respectively as high as 15200mg / L and 7700mg / L have brought huge pressure to the environment and become a bottleneck restricting the development of the soybean product industry. How to deal with huge pollution is an important problem to be solved urgently. [0003] Tofu wastewater is tofu yellow slurry water, which is the wastewater that flows out when the soybean products are pressed and formed after the dots are solidified. The color is yellow and slightly green, and th...

Claims

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Application Information

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IPC IPC(8): A23J1/16A23L1/305A23L2/66A23L33/185
Inventor 孙培龙孟祥河邵平褚绍霞
Owner ZHEJIANG UNIV OF TECH
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