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Apparatus for simultaneously detecting 10 kinds of food microorganism and screening diagnosis method thereof

A diagnostic method and microbial technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to sterilize, exceed the standard of microbial indicators, and single detection types, so as to prevent cross-contamination of samples, easy to operate, and convenient to move Effect

Inactive Publication Date: 2009-07-01
万积成
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The student soy milk poisoning incident in Jilin City in 2001 was because the production company changed the process parameters without authorization and lowered the sterilization temperature from 82°C to 60°C. , leading to food poisoning of a large number of students
The type of detection of this patented technology is too single, and it is of little significance to promote and use it on a large scale

Method used

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  • Apparatus for simultaneously detecting 10 kinds of food microorganism and screening diagnosis method thereof
  • Apparatus for simultaneously detecting 10 kinds of food microorganism and screening diagnosis method thereof
  • Apparatus for simultaneously detecting 10 kinds of food microorganism and screening diagnosis method thereof

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Experimental program
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Embodiment Construction

[0042] figure 2 It is an exploded view of the test paper. It can be seen from the figure that the detection test paper consists of a bottom plate (7), a water-absorbing plate (1), a nitrocellulose membrane (2), a color particle fixing pad (5), a sample liquid-absorbing layer (6), and a MAX line ( 8) Composition; the middle part of the bottom plate (7) is a nitrocellulose membrane (2), and there is a test line (4) and a polyclonal antibody control line (3) on the nitrocellulose membrane (2), and one end of the bottom plate is a water-absorbing The other end is a sample liquid-absorbing layer; the two ends of the nitrocellulose membrane are respectively overlapped with the water-absorbing plate and the monoclonal antibody storage pad, and the sample liquid-absorbent layer is pressed on the monoclonal antibody storage pad.

[0043] The test paper can complete a simultaneous detection of 10 kinds of food microorganisms through the sample cup:

[0044] Depend on Figure 5 As sho...

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Abstract

The invention relates to a device for checking 10 food microbes synchronously and a screen diagnosis method, which adopts check test paper and a sample cup to process one-time synchronous check on 10 food microbes. The invention is characterized in that an immunity method is selected to select two matched monoclonal antibodies for different antigenic determinants of one strain as a food microbe antibody I and a food microbe antibody II; the invention utilizes prepared matched monoclonal antibodies and utilizes color particle immunity chromatography method to prepare test paper; the 10 food microbe test paper are arranged in one sample cup, and when adds a liquid sample, the one displays two read lines is considered as the existence of one microbe. The invention combines general food microbe agents together, to detect 10 microbes for one sample, thus being convenient for screening and on-time diagnosis.

Description

technical field [0001] The invention relates to a movable, convenient and practical test paper and a screening diagnosis method for simultaneously detecting 10 kinds of food microorganisms prepared by color particle immunochromatography, which can detect multiple samples at the same time. In particular, it has the characteristics of quantitative sampling and scientific sampling. Background technique [0002] "Food is paramount to the people." In our country with a large population, food issues, especially food safety issues, are more important. It may be due to various reasons, such as backward food production conditions, irregular operations in the production process, etc., resulting in unqualified food. This has just brought very big hidden danger to people's life health. One of the most important indicators of unqualified food is microbial exceeding the standard. The student soy milk poisoning incident in Jilin City in 2001 was because the production company changed th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569
Inventor 万积成
Owner 万积成
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