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Method for extracting collagen protein small peptide freeze-dried powder from skin deep-sea cod

A technology of collagen and deep-sea cod, which is applied in the field of deep-sea cod skin extraction collagen small peptide freeze-dried powder, can solve the problems of waste of deep-sea biological resources, etc., and achieve the effect of small molecular distribution range, wide application and high purity of small peptides

Inactive Publication Date: 2011-08-31
大连隆合海洋生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most cod skins are treated as crude protein feed, resulting in a waste of deep-sea biological resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 100kg of fresh cod skin to remove impurities, put it into a 1000L open soaking tank, add 400L of edible water, and soak at a temperature of 20°C for 8 hours. Then fish out the fish under the skin with a knife, scrape off the attachments on the surface of the fish skin, and rinse with drinking water. Then the 100Kg cod skin soaked in water. Put it into a 1000L rehydration mixing tank and add 2% Ca(OH) 2 400L aqueous solution, controlled temperature at 20°C, filled with compressed air of 50pa to generate a large amount of water bubbles, maintained for 8 hours, removed and drained, minced fish skin with a meat grinder, added 400L of pure water and stirred evenly, passed Colloid mill cut into fish skin slurry.

[0032] Vacuum the pretreated cod skin slurry into the bio-enzyme reactor, heat it to 38°C, adjust the pH value to 7, start the agitator, and add 200g of compound protease, 600g of AS1398 neutral protease, and 1203 fat in an orderly manner Enzyme 100g, lactob...

Embodiment 2

[0037] The difference from Example 1 is: remove 100 kg of frozen cod skin, soak it in 200 kg of drinking water for 12 hours at 18 ° C, remove the fish meat of the poncho with a skin scraper, clean it, and then put it into the air-water mixing tank , adding a concentration of 3% Ca(OH) 2200L aqueous solution, control the temperature at 18°C, fill it with 200pa compressed air, generate bubbles in the water tank, keep it for 12 hours, take out the cod skin and drain it, mince it with a meat grinder, add 200kg of pure water, and pass it through a colloid mill Cut into pulp.

[0038] Use vacuum to suck into the 500L bioreactor, control the temperature at 58°C, the pH value is 7, and the vacuum degree is 200pa. Start the agitator, add 300g of compound protease, 300g of AS 1 398 neutral protease, 200g of 1203 lipase, and lactic acid bacteria during stirring. Su 40g, Vc400g. Composite enzymatic hydrolysis for 10 hours, after filtering, put it into the modification reaction kettle, t...

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PUM

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Abstract

The invention relates to the deep processing of deep-sea cod, specifically a method for extracting collagen small peptide freeze-dried powder from deep-sea cod skin, comprising the following steps: 1) pre-treating deep-sea cod skin: soaking the fresh or frozen cod skin, decomposing into fluid for reservation; 2) biological enzymolysis; 3) separating and purifying protein small peptide; 4) freezing and drying to get the collagen small peptide freeze-dried powder extracted from deep-sea cod skin. The invention provides a wider use range for the biological protein in waste cod skin.

Description

technical field [0001] The invention relates to deep processing of deep-sea cod, in particular to a method for extracting collagen small peptide freeze-dried powder from deep-sea cod skin. Background technique [0002] During the deep processing of deep-sea cod skin, 16%-20% of the remaining material becomes waste. Cod skin contains high collagen protein, and this protein can promote the growth of human epidermal and dermal tissue cells, and can also Rich in human skin elasticity and vitality. However, most cod skins are treated as rough protein feed, resulting in a waste of deep-sea biological resources. After research, deep-sea cod skin is used as raw material, and small collagen peptides are extracted using marine biological core technology. The added value of the cod skin is improved, and a new way is also opened up for the cod skin to be a high-grade nutritious food. Contents of the invention [0003] The purpose of the present invention is to provide a preparation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/04
Inventor 李长青叶永和李梅李晓龙李丹丹王丹
Owner 大连隆合海洋生物工程技术有限公司
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