A kind of deer blood deep processing method
A technology of deep processing and deer blood, which is applied in the field of animal blood processing, can solve the problems of restricting the consumption of deer blood, and achieve good application prospects, simple and feasible reaction methods, and simple and feasible methods
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Embodiment 1
[0027] Using fresh deer blood as raw material, deer blood peptides are prepared according to the following process:
[0028] Take 1 liter of fresh deer blood and centrifuge at 4°C and 3000 rpm for 2 hours to precipitate blood cells for the preparation of SOD and heme, and the supernatant is deer blood plasma protein for the preparation of active peptides.
[0029] (1) Preparation of SOD and heme
[0030] Add 1 liter of deionized water to the blood cells and let it stand overnight, then add 0.5 liters of ethanol / chloroform mixture with a volume ratio of 1:3 of deionized volume, stir for 10 minutes, let it stand still, and centrifuge at 1000 rpm for 10 minutes , the supernatant was heated at 80°C for 10 minutes and then centrifuged for 10 minutes. The supernatant was crude SOD, and the precipitate was crude heme.
[0031] Add 2 liters of acetone to the crude product of SOD, stir and let stand for 5 minutes to filter, add 2 liters of distilled water to dissolve the precipitate a...
Embodiment 2
[0036] Take 1 liter of fresh deer blood and centrifuge at 4°C and 15,000 rpm for 0.1 hour to precipitate blood cells for the preparation of SOD and heme, and the supernatant is deer blood plasma protein for the preparation of active peptides.
[0037] (1) Preparation of SOD and heme
[0038]Add 1 liter of deionized water to the blood cells and let it stand overnight, then add 0.5 liters of ethanol / chloroform mixture with a volume ratio of 1:4, stir for 50 minutes, let it stand still, and centrifuge at 10,000 rpm for 10 minutes, and the supernatant After heating at 50°C for 50 minutes and centrifuging for 10 minutes, the supernatant is crude SOD, and the precipitate is crude heme.
[0039] Add 2 liters of acetone to the crude SOD and stir it, then let it stand for 5 minutes and filter, add 2 liters of distilled water to dissolve the precipitate, then centrifuge at 10,000 rpm for 10 minutes, freeze-dry the supernatant, dissolve it with 1M, pH=9 phosphate buffer, and put it on Se...
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