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Composite microbial inoculant for corn silage and use method thereo

A technology of compound microorganisms and corn silage, which is applied in the field of corn silage inoculants, can solve the problems of secondary fermentation and corn silage instability, and achieve the effect of maintaining stable pH value, slow value increase, and increasing milk production and milk fat rate

Inactive Publication Date: 2009-06-10
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the problem that the existing silage inoculant mainly based on homozyme fermentation makes corn silage unstable after opening the cellar and easily produces secondary fermentation, and provides a corn silage that can improve the preservation effect of corn silage. Composite microbial inoculant for silage and method of use thereof

Method used

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  • Composite microbial inoculant for corn silage and use method thereo
  • Composite microbial inoculant for corn silage and use method thereo
  • Composite microbial inoculant for corn silage and use method thereo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of inoculant

[0020] The ratio of raw materials used is as follows:

[0021] Lactobacillus Brucella freeze-dried powder: 1 part by weight

[0022] Lactobacillus plantarum freeze-dried powder: 2 parts by weight

[0023] Skim milk powder: 30 parts by weight

[0024] Glucose: 60 parts by weight

[0025] Manganese sulfate: 7 parts by weight

[0026] Fully mix the lactic acid bacteria freeze-dried powder and the nutrient mixture evenly, pass through a 40-mesh sieve to obtain a powder with good fluidity, and the total number of Lactobacillus Brucella in the compound inoculum is 2×10 9 ~5×10 9 c Total number of Lactobacillus plantarum 5×10 9 ~7.5×10 9 cfu / g. Packed in paper-plastic or composite aluminum foil bags, the storage temperature is less than 35°C, the humidity of the storage environment is less than 60%, and the survival rate of lactic acid bacteria within one year is greater than 90%.

[0027] (2) How to use

[0028] The whole plant corn is...

Embodiment 2

[0030] (1) Preparation of inoculant

[0031] The ratio of raw materials used is as follows:

[0032] Lactobacillus Brucella freeze-dried powder: 2 parts by weight

[0033] Lactobacillus plantarum freeze-dried powder: 2 parts by weight

[0034] Skim milk powder: 30 parts by weight

[0035] Glucose: 58 parts by weight

[0036] Manganese sulfate: 8 parts by weight

[0037] Fully mix the lactic acid bacteria freeze-dried powder and the nutrient mixture evenly, pass through a 40-mesh sieve to obtain a fluid-type powder, and the total number of Lactobacillus Brucella in the compound inoculant is 5×10 9 ~8×10 9 cfu / g, the total number of Lactobacillus plantarum 7.5×10 9 ~1×10 10 cfu / g. Packed in paper-plastic or composite aluminum foil bags, the storage temperature is less than 35°C, the humidity of the storage environment is less than 60%, and the survival rate of lactic acid bacteria within one year is greater than 90%.

[0038] (2) How to use

[0039] Same as the method ...

Embodiment 3

[0041] (1) Preparation of inoculant

[0042] The ratio of raw materials used is as follows:

[0043] Lactobacillus Brucella freeze-dried powder: 3 parts by weight

[0044] Plantarum lyophilized powder: 4 parts by weight

[0045] Skimmed milk powder: 31 parts by weight

[0046] Glucose: 51 parts by weight

[0047] Manganese sulfate: 10 parts by weight

[0048]Fully mix the lactic acid bacteria freeze-dried powder and the nutrient mixture, and pass through a 40-mesh sieve to obtain a fluid-type powder. The total number of Lactobacillus Brucella in the compound inoculant is 8×10 9 ~1×10 10 cfu / g, total number of Lactobacillus plantarum 1×10 10 ~2×10 10 cfu / g. Packed in paper-plastic or composite aluminum foil bags, the storage temperature is less than 35°C, the humidity of the storage environment is less than 60%, and the survival rate of lactic acid bacteria within one year is greater than 90%.

[0049] (2) How to use

[0050] Same as the method of use in Example 1.

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Abstract

The invention discloses an inoculant containing homofermentation malolactic bacteria and heterofermentation malolactic bacteria for corn silage and using method thereof. The composite microorganism inoculant for corn silage includes 2*10<9>cfu / g-5*10<10>cfu / g of lactobacillus buchneri and 5*10<9>cfu / g-1*10<11>cfu / g of lactobacillus plantarum. The composite microorganism inoculant for corn silage provided by the present invention employs the homofermentation malolactic bacteria and the heterofermentation malolactic bacteria as acid generation source, thereby increasing preservation effect of corn silage forage. When silage forage prepared from the inoculant of the present invention is used as forage of cow, milk yield and butterfat rate can be increased.

Description

technical field [0001] The invention relates to an inoculant for silage, in particular to an inoculant for corn silage containing both homofermentative lactic acid bacteria and heterofermentative lactic acid bacteria and its application method. Background technique [0002] With the continuous improvement of people's living standards and the adjustment of rural industrial structure, the production of herbivorous livestock such as cattle and sheep has achieved unprecedented development. One of the outstanding problems encountered in the development of the herbivorous livestock industry at present is that the supply of high-quality roughage is seriously insufficient, resulting in poor health of livestock, high feeding costs and low economic benefits. [0003] Silage has become the main source of roughage for ruminants such as cattle and sheep because of its high nutritional value, low processing cost and not being affected by seasons. The proportion of silage in dairy cow feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/02A23K30/18
Inventor 傅彤刁其玉高腾云时建忠孙宇李改英
Owner HENAN AGRICULTURAL UNIVERSITY
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