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Primer for detecting sulfonylureas receptor 1 gene polymorphism sites, the application thereof and kits thereof

A technology of polymorphic sites and gene polymorphism, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve problems such as individual differences, affecting the efficacy of drugs, and affecting the occurrence of diseases, so as to control blood sugar, The effect of reducing medical costs and reducing side effects

Active Publication Date: 2009-06-03
深圳泰乐德医疗有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Genetic background may not only affect the occurrence of diseases, but also individual differences in drug efficacy

Method used

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  • Primer for detecting sulfonylureas receptor 1 gene polymorphism sites, the application thereof and kits thereof
  • Primer for detecting sulfonylureas receptor 1 gene polymorphism sites, the application thereof and kits thereof
  • Primer for detecting sulfonylureas receptor 1 gene polymorphism sites, the application thereof and kits thereof

Examples

Experimental program
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Embodiment 1

[0029] Example 1 Detection of SUR1 gene T1369G polymorphism genotype by PCR method

[0030] 1. Sample preparation

[0031] Twelve EDTA anticoagulants were taken as the sample source, and the samples were prepared according to the following steps.

[0032] 1. In a 1.5ml centrifuge tube, add 100μl of EDTA anticoagulant specimen

[0033] 2. Add 300 μl of erythrocyte lysate and incubate at 37°C for 20 minutes.

[0034] 3. Centrifuge and take the pellet

[0035] 4. Add 100 μl leukocyte lysate, resuspend the pellet, and incubate at 37°C for 15 minutes

[0036] 5. Add 35 μl of protein precipitation solution and shake at high speed for 90 seconds.

[0037] 6. Centrifuge, take the supernatant, transfer all the supernatant into a 1.5ml centrifuge tube containing 100μl isopropanol, shake gently back and forth 10 times until white flocs appear; centrifuge at 15000g for 90 seconds, the bottom of the centrifuge tube A white precipitate was visible.

[0038] 7. Discard the supernatant,...

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Abstract

The invention provides an auele specific primer for detecting SUR1 gene T1369G polymorphism sites (rs757110) genotype by a PCR method, the application of the auele specific primer in preparing a reagent for detecting SUR1 gene T1369G polymorphism sites by a PCR augmentation biological sample, a SUR1 gene T1369G polymorphism site detecting kit containing auele specific primers and the application of the detecting kit in predicting drug effect of sulfonylureas. The medicines of the sulfonylureas are selected from glibenclamide, glibornuride, glycyclamide, glyhexamide, glimepiride, glypinamide, glisamuride, glisentide, glisolamide, glyoctamide, gliclazide, glipizide and gliquidone. The selective preference is gliclazide or glimepiride.

Description

technical field [0001] The invention relates to a specific primer for detecting the genotype of a sulfonylurea receptor gene polymorphism site by a PCR method, especially a specific primer for the polymorphic site of the sulfonylurea receptor 1 gene T1369G (rs757110), And the use of the specific primer in the preparation of a reagent for detecting the T1369G polymorphic site of the sulfonylurea receptor 1 gene by PCR amplifying a biological sample, and the polymorphic site of the sulfonylurea receptor 1 gene T1369G comprising the specific primer The invention relates to a point detection kit and the use of the kit for predicting the effect of sulfonylurea drugs, belonging to the field of medical biotechnology. Background technique [0002] Sulfonylurea antidiabetic drugs are insulin secretagogues that lower fasting and postprandial blood sugar. For most newly diagnosed patients with type 2 diabetes, sulfonylurea antidiabetic drugs can reduce fasting blood glucose by 50-80 m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 徐希平王燕王玉张彦明谢爱武于多邢厚恂
Owner 深圳泰乐德医疗有限公司
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