In-vitro tender end container seedling formation method for cluke cherry dwarfing rootstock

A technology for sweet cherry and dwarfing stock, which is applied in the field of the method for growing young shoots from the dwarfing stock of sweet cherry, an excellent rootstock variety of fruit trees, can solve the problems of high breeding cost and difficulty in the breeding of sweet cherry dwarfing stock, and achieves improved reproduction speed, The effect of improved seedling quality and strong disease resistance

Active Publication Date: 2009-05-20
SHANDONG INST OF POMOLOGY
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Problems solved by technology

[0003] In order to solve the difficult problem of high cost and difficulty in the breeding of sweet cherry dwarfing stock, the present invention provides a method for growing seedlings of sweet cherry dwarfing stock in vitro and young shoots in containers, and the technical scheme adopted is:

Method used

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with embodiment:

[0017] This seedling-forming method comprises the following steps:

[0018] (1) Young shoots harvested and cut

[0019] From June to October every year, select dwarf stock seedlings of sweet cherry with vigorous branches, use a sterilized tip cutting tool to cut 15-20cm long young shoots at the top of the branches, remove and dip the leaves at the bottom 5-10cm of the young shoots Rooting liquid, the formula of rooting liquid is MS vitamins + 1000ppm IBA + 1000ppm NAA, and the pH value of rooting liquid is between 5.4-5.8. Pick and cut young shoots every day from 6 am to 8 am, and pick leaves under shady conditions.

[0020] Tip cutting tools are subject to partial contact high-temperature disinfection at a temperature of 300°C, and each contact disinfection time is 15 seconds.

[0021] (2) Rapid rooting in the isolated young shoot container

[0022] The bottom of the tender shoo...

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Abstract

The invention discloses an in vitro young-shoot container seedling formation method for cherry dwarfing rootstock. The method comprises the steps of collecting and shearing young shoots, allowing in vitro young shoots to root rapidly in a container, transplanting new seedlings and the like. Compared with the conventional tissue culture seedling cultivation method, the method can ensure that the rooting rate of the in vitro young shoots of the cherry dwarfing rootstock rises to more than 90 percent from 50 percent; the survival rate of the new seedlings after transplantation rises to more than 98 percent from 60 percent; and the new seedlings after field planting have no seedling recovering period, and are fast to grow and strong in disease resisting capability. The method is suitable for large-area popularization in China.

Description

technical field [0001] The invention relates to a method for growing seedlings of sweet cherry dwarfed rootstocks and tender shoots of an excellent rootstock variety of fruit trees, in particular to a method for growing seedlings of sweet cherry dwarfed rootstocks in vitro and young shoots in containers. Background technique [0002] Sweet cherry dwarfing stock is a clone intermediate rootstock, which has good grafting compatibility with most cherry varieties. It has the obvious advantages of dwarfing, high yield, cold resistance, disease resistance, and waterlogging tolerance, and it is an urgently needed dwarf rootstock variety for fruit trees in China. However, because it is a clonal rootstock, it cannot be propagated by seeds, and it is very difficult to take root by conventional layering and hardwood cuttings. At present, the domestic method of seedling cultivation is mainly adopted by tissue culture, and its cost is very high, which seriously limits the promotion of sw...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01G31/00A01G9/10
Inventor 刘庆忠张道辉魏海蓉艾呈祥李国田
Owner SHANDONG INST OF POMOLOGY
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