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Environmental microorganism detection method and system

A technology of environmental microorganisms and detection methods, which is applied in the direction of microbial measurement/inspection, biochemical equipment and methods, special data processing applications, etc., and can solve problems such as difficult to identify trace species

Inactive Publication Date: 2009-05-13
SHENZHEN HUADA GENE INST +1
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  • Abstract
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for detecting environmental microorganisms, aiming to solve the problem that the existing environmental microorganism detection methods are difficult to identify trace species

Method used

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  • Environmental microorganism detection method and system
  • Environmental microorganism detection method and system
  • Environmental microorganism detection method and system

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Embodiment Construction

[0021] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] In the embodiment of the present invention, high-throughput sequencing technology is used to sequence the DNA extracted from environmental samples to obtain a DNA tag sequence. After removing possible carrier contamination in the DNA tag sequence, the DNA tag sequence is combined with the existing The known sequences in the known database are compared to obtain the classification of the DNA tag sequence.

[0023] figure 1 The implementation process of the environmental microorganism detection method provided by the embodiment of the present invention is shown, and the details are as follows...

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Abstract

The invention is suitable for the field of bioengineering and provides a method and a system for detecting environmental microorganisms. The method comprises the following steps: DNA extracted from an environmental sample is sequenced by the high-flux sequencing technology, and DNA label sequence is obtained; vector pollution in the DNA label sequence is removed; and the DNA label sequence is compared with the known sequence in a known database, and the class of the DNA label sequence is determined according to the comparison result. The embodiment of the method can detect possible microbial species and possible varieties of microbial species in the environmental sample.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to an environmental microorganism detection method and system. Background technique [0002] Both proteins and RNA molecules that determine biological traits store information in biological cells in the form of coding sequences of the four bases of DNA. This DNA molecule contains the complete set of genetic information of an organism. In order to understand the function and role of genetic information from an overall perspective, the most important step is to determine the complete set of genetic information of the organism, that is, to know the sequence of all DNA bases of the organism. Traditional genome sequencing mainly uses the "Sanger" sequencing technology, also known as the "terminal termination" sequencing technology. The biggest disadvantage of this sequencing method is: high cost and low yield. In recent years, the "next-generation high-throughput sequencing technology" r...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G06F19/00G06F19/22
Inventor 覃俊杰李瑞强张秀清王俊杨焕明汪建
Owner SHENZHEN HUADA GENE INST
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