Method for cultivating hon-shimeji mushroom on fungal bed
一种栽培方法、离褶伞的技术,应用在真姬离褶伞的菌床栽培领域,能够解决麦类价格高、没有达到商业生产、培养基成本高等问题,达到经济性优良的效果
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[0049] In 100mL PGY liquid medium (composition: glucose 2.0% (w / v), peptone 0.2% (w / v), yeast extract 0.2% (w / v), KH 2 PO 4 0.05% (w / v), MgSO 4 ·7H 2 O 0.05% (w / v)) to inoculate the hyphae of Lyophyllum shjmeji La 01-27 strain (FERMBP-10960), shake culture (100rpm) at 25°C for 7 days, implant 2mL into 200mL of the same medium, continue After shaking culture (100rpm) for 7 days, the whole amount of the culture was inoculated into a 200-L fermenter (Ja-Fa-Menta-) (Komatsukawa Works) added with 160L of the same medium, and stirred for 6 days ( Stirring speed: 100rpm, ventilation rate 25L / min), to produce liquid inoculum. On the other hand, mix crushed corn (manufactured by Iizaka Seimai Co., Ltd.) with coniferous sawdust cedar powder [(Yu) Tomoe Bussan] at a dry matter weight ratio of 2:1 (crushed corn: coniferous sawdust), add water and stir well , The culture medium mixed until the water content of the medium is finally 62% by weight is placed in a batch of about 5000 polyp...
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