Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material
A crude polysaccharide and gastrodin alcohol technology, applied in plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., to achieve complete extraction, easy absorption, and improved physiological effects
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Embodiment 1
[0011] Wash 60kg of fresh Gastrodia elata and then use microwave method to kill green, and the heart of Gastrodia elata shall prevail;
[0012] Crush Gastrodia elata after killing green, add water to refine pulp, and perform centrifugal filtration twice in different ways, that is, coarse filtration with three-legged centrifuge and fine filtration with tubular centrifuge. Add 95% ethanol with three times the volume ratio to the supernatant under stirring, and statically precipitate Gastrodia elata The crude polysaccharide is filtered to take out the Gastrodia elata crude polysaccharide, dried and crushed under normal pressure and a temperature of 50°C to obtain Gastrodia elata crude polysaccharide powder;
[0013] The Gastrodia elata filter residue is percolated and extracted with the alcohol-water clear liquid after the polysaccharide is precipitated, and the extraction parameters of the gastrodia elata ethanol are: the ethanol concentration of the alcohol-water clear liquid is...
Embodiment 2
[0015] Wash the fresh Gastrodia elata and use the steaming method to complete the greening, and the heart of Gastrodia elata shall prevail;
[0016] Crush Gastrodia elata after killing green, add water to refine pulp, and perform centrifugal filtration twice in different ways, that is, coarse filtration with three-legged centrifuge and fine filtration with tubular centrifuge. Add 95% ethanol with three times the volume ratio to the supernatant under stirring, and statically precipitate Gastrodia elata The crude polysaccharide is filtered to take out the Gastrodia elata crude polysaccharide, dried and crushed under vacuum at a temperature of 65°C to obtain Gastrodia elata crude polysaccharide powder;
[0017] The Gastrodia elata filter residue is percolated and extracted with the alcohol-water clear liquid after the polysaccharide is precipitated, and the extraction parameters of the gastrodia elata ethanol are: the ethanol concentration of the alcohol-water clear liquid is 75%,...
Embodiment 3
[0019] Wash the fresh Gastrodia elata and use the microwave method to complete the greening, and the heart of Gastrodia elata shall prevail;
[0020] Crush Gastrodia elata after killing green, add water to refine pulp, and perform centrifugal filtration twice in different ways, that is, coarse filtration with three-legged centrifuge and fine filtration with tubular centrifuge. Add 95% ethanol with three times the volume ratio to the supernatant under stirring, and statically precipitate Gastrodia elata The crude polysaccharide is filtered to take out the Gastrodia elata crude polysaccharide, dried and crushed under vacuum at a temperature of 70°C to obtain Gastrodia elata crude polysaccharide powder;
[0021] The Gastrodia elata filter residue is percolated and extracted with the alcohol-water clear liquid after the polysaccharide is precipitated, and the extraction parameters of the gastrodia elata ethanol are: the ethanol concentration of the alcohol-water clear liquid is 72%...
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