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Method for quickly breeding Malus 'Hongying' by tissue culture

A technology of tissue culture and Begonia, which is applied in the field of tissue culture and fast-propagating seedlings of Begonia chinensis, and can solve problems such as the lack of Begonia chinensis.

Inactive Publication Date: 2009-01-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, although there have been patents and reports on the tissue culture seedling propagation technology of many tree species at home and abroad, because the red tasseled crabapple is a new forest tree species, there is no literature report on the tissue culture of the red tasseled crabapple at present.

Method used

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  • Method for quickly breeding Malus 'Hongying' by tissue culture
  • Method for quickly breeding Malus 'Hongying' by tissue culture
  • Method for quickly breeding Malus 'Hongying' by tissue culture

Examples

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Embodiment Construction

[0072] a. Material collection--select excellent mother plants of Begonia chinensis, and select new shoots free of diseases and insect pests as explants at around 11:00 noon on a sunny day from March to April.

[0073] b. Aseptic treatment--cut the harvested branches into small sections with terminal buds and lateral buds of about 2.0 cm, cut off the leaves and petioles, first use alcohol absorbent cotton to gently scrub the fluff on the explants, and then place them Soak in 10% detergent solution for 30 minutes, then rinse with running water for 4 hours. Under sterile conditions, soak in 75% alcohol for 30 seconds, then treat with 0.1% mercury liter for 10 minutes, rinse with sterile water for 3 to 5 times, and finally absorb residual water with sterile filter paper.

[0074] c. Inoculation start-up culture--on the ultra-clean workbench, inoculate the sterilized stem segments (with axillary buds or terminal buds) into the start-up medium N6+BA0.50mg L -1 +NAA0.10mg·L -1 +Suc...

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Abstract

The invention relates to a method for tissue culture and rapid propagation of red-tassel crabapple and is characterized in that an annual shoot of excellent mother plant of the red-tassel crabapple is taken as an explant, then the seedling can be obtained after asepsis, inoculation starting culture, differentiation culture, propagation culture, rooting culture and acclimatization and transplanting. N6 basic medium is used in the inoculation starting culture, the differentiation culture and the propagation culture, and the rooting culture uses 1 / 2 N6 basic medium, major elements of which is halved. The propagation coefficient of the propagation seedling of the red-tassel crabapple by the method is above 8, thus relieving the conflict of seedling lack of new breed greatly.

Description

1. Technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture in forestry, and in particular relates to a production method for rapidly propagating seedlings of Begonia chinensis through tissue culture. 2. Background technology [0002] Malus ‘Hongying’, a small deciduous tree belonging to the genus Malus in the family Rosaceae, is a new variety of Malus ‘Hongying’ selected and bred by Nanjing Forestry University in 2007. Begonia is a plant of the Rosaceae (Rosaceae) genus Malus. It is a small deciduous tree or shrub. Very few species grow in subtropical regions and are semi-evergreen trees. They are distributed across Eurasia and North America, from 20° north latitude to in the area between 50°. There are about 50 species in the world, and there are about 23 species in my country, 16 of which are endemic species. The common ones are Malus halliana, Malus prunifolia, Malus hupehensis, and Xifu ( Malus micromalus) and so ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 沈永宝游小英
Owner NANJING FORESTRY UNIV
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