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Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid

A technology of gulonic acid and stability, applied in the field of bioengineering, can solve the problems of unstable yield and fermentation cycle

Inactive Publication Date: 2009-01-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

During vitamin C fermentation, differences between batches of corn st

Method used

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  • Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid
  • Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid
  • Method for enhancing fermentation and production stability of 2-ketone-L-Gulonic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0043] The influence of embodiment 1 three kinds of mixed solutions on vitamin C fermentation

[0044] Orthogonal experiments were used to investigate the important role of metal ion mixture, amino acid mixture and vitamin mixture in vitamin C fermentation. Follow L 25 (5 3 ) Orthogonal test factor level table arranges an orthogonal test, wherein the levels of various mixed solutions are multiples of the content of each amino acid, vitamin, and metal ion mixed solution in corn steep liquor. The specific experimental design and results are shown in Table 4 and Table 5.

[0045] Form 4L 25 (5 3 ) Orthogonal test factor level table

[0046] factor / level

[0047] Form 5L 25 (5 3 ) Orthogonal experimental design and experimental results

[0048]

[0049] 8

[0050] According to the method of Montgomery (1991), the results of the orthogonal experiment were visually analyzed, the results are as follows figure 1 shown. It can be seen that in t...

Embodiment 2

[0051] The influence of embodiment 2 metal ions on vitamin C fermentation

[0052] On the basis of embodiment 1, according to the effect of metal ion on the key enzyme L-sorbose dehydrogenase of KGA synthesis pathway, be divided into three groups: (1) the ion that L-sorbose dehydrogenase is activated: Mg 2+ , Ca 2+ , Zn 2+ ; (2) the ions that inhibit L-sorbose dehydrogenase: Cu 2+ , Mn 2+ , Fe 2+ ,Co 2+ ; (3) Other ions: K + , Na + , Cr 2+ . P is added to the third type of metal ions and examined together. Orthogonal experiments were also used to investigate the important role of these three types of metal ions in the synthesis of vitamin C. Follow L 16 (4 3 ) Orthogonal test factor level table arranges the orthogonal test, the content of amino acid and vitamin is 5 times and 1 time respectively. The specific experimental design and results are shown in Table 6 and Table 7.

[0053] Form 6L 16 (4 3 ) Orthogonal test factor level table

[0054] factor / ...

Embodiment 3

[0058] Embodiment 3 The impact of the first group of metal ions on vitamin C fermentation

[0059] On the basis of Example 2, the first group of metal ions (Mg 2+ , Ca 2+ , Zn 2+ ) plays an important role in KGA synthesis. Follow L 16 (4 3 ) Orthogonal test factor level table arranges the orthogonal test. The specific experimental design and results are shown in Table 8 and Table 9.

[0060] Form 8L 16 (4 3 ) Orthogonal test factor level table

[0061] factor / level

[0062] Form 9L 16 (4 3 ) Orthogonal experimental design and experimental results

[0063]

[0064] The results of the orthogonal test were visually analyzed, the results are as follows: image 3 shown. It can be seen that in the selected concentration range: (1) increase Mg 2+ Concentration can promote KGA synthesis, but has no significant effect on cell growth and L-sorbose consumption; (2) increase Ca + , Zn 2+ The concentration had no significant effect on cell growth, KGA sy...

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Abstract

A method for improving the stability for the fermentation production of 2-keto-L-gulonic acid belongs to the technical field of bioengineering. The 2-keto-L-gulonic acid is also named as vitamin C. The invention uses three mixture liquids to replace corn pulp, adopts an orthogonal test to investigate the important effects of a metal ion mixture liquid, an amino acid mixture liquid and a vitamin mixture liquid in the fermentation of the vitamin C. Based on the obtaining that the metal ion mixture liquid is the most remarkable affecting factor for cell growing, KGA synthesizing and L-sorbose consumption, the method of the invention further adopts the orthogonal test to investigate the effects of various metal irons to the fermentation of the vitamin C and discovers that increasing the concentration of Mg<2+> can promote the synthesizing of the KGA and relates to the most remarkable affecting factor for the fermentation of the vitamin C. The output and yield of the KGA which adopts optimized combination to carry out the fermentation of the vitamin C are respectively to be 75.2g/L and 0.94g/g. Compared with a common culture medium, the output (67g/L) and the yield (0.84g/g) of the KGA are respectively improved by 12.24 percent and 11.9 percent.

Description

technical field [0001] A method for improving the stability of 2-keto-L-gulonic acid fermentation production belongs to the technical field of bioengineering. 2-keto-L-gulonic acid (2-keto-L-gulonic acid, KGA) is also called vitamin C, abbreviated as KGA, and the present invention relates to a key influencing factor of vitamin C fermentation. By studying the influence of trace element solution (metal ions, vitamins, amino acids) on the fermentation of vitamin C, the most significant factors affecting the fermentation of vitamin C were obtained. Background technique [0002] Vitamin C, also known as ascorbic acid, as an essential vitamin and antioxidant, is widely used in pharmaceutical, food, beverage, cosmetic and feed industries. [0003] With the continuous expansion of the application range of vitamin C, the market demand for vitamin C is also increasing. The earliest process to realize the industrial production of vitamin C was the five-step chemical reaction and one-...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P17/04C12R1/11C12R1/01
Inventor 刘立明张静陈坚刘杰
Owner JIANGNAN UNIV
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