Method for determining citric acid concentration and citric acid determining reagent kit

A technology of citric acid and kits, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral characteristic measurement, etc., and can solve the problems of impurities in finished products and incomplete conversion of calcium carbonate

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the conversion of raw material calcium car...

Method used

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Experimental program
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Effect test

Embodiment 1

[0035] The citric acid determination reagent of the present embodiment is a single reagent, comprising:

[0036] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0037] Stabilizer 500mmol / L

[0038] Reduced coenzyme 0.25mmol / L

[0039] Citrate lyase 10000U / L

[0040] Malate dehydrogenase 12000U / L

[0041] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0042] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured citric acid sample to the reagent is 1 / 25 , the reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0043] After adding the sample and reagent, let them mix and react...

Embodiment 2

[0045] The citric acid determination reagent of the present embodiment is double reagent, comprises:

[0046] Reagent 1

[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 50mmol / L

[0049] Reduced coenzyme 0.25mmol / L

[0050] Reagent 2

[0051] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0052] Stabilizer 500mmol / L

[0053] Citrate lyase 10000U / L

[0054] Malate dehydrogenase 12000U / L

[0055] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0056] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured citric acid sample to reagent 1 and reagent 2 It is 2 / 20 / 5, the reaction direction is negative reaction (downward reaction), the delay time is about 0 minute...

Embodiment 3

[0059] The citric acid determination reagent of the present embodiment is three reagents, comprises:

[0060] Reagent 1

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Reduced coenzyme 0.25mmol / L

[0064] Reagent 2

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 500mmol / L

[0067] Malate dehydrogenase 12000U / L

[0068] Reagent 3

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 500mmol / L

[0071] Citrate lyase 10000U / L

[0072] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0073] When measuring the concentration of citric acid, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, test citric ac...

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PUM

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Abstract

The invention relates to a kit for diagnosing/mensurating citric acid by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the citric acid, and belongs to the technology field of environment/food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, citrate cleavage enzyme,malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet/visible light analyzer, and the degree/velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the citric acid.

Description

technical field [0001] The invention relates to a citric acid determination kit, and at the same time, the invention also relates to a method for determining the concentration of citric acid, which belongs to the technical field of food / environmental testing and determination. Background technique [0002] Citric acid is mainly used in the food and beverage industry as sour agent, flavoring agent, preservative, and preservative, and is also used in the preparation of pharmaceutical cooling agents. It is also used as an antioxidant, plasticizer, detergent. [0003] The mandatory national standard GB17203-1998 "Food Additive Calcium Citrate" stipulates that the content of calcium citrate is 98.0-100.5%. The General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China organized a national supervision and spot check on the product quality of food additive citric acid and its salts (citric acid, potassium citrate, sodium citrate, cal...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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