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Endotoxin absorbent and preparation thereof

An adsorbent and endotoxin technology, applied in chemical instruments and methods, suction devices, and other chemical processes, can solve problems such as risk of use, easy generation of particles, and large damage to the strength of agarose gel

Active Publication Date: 2008-12-17
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such conditions greatly damage the strength of the agarose gel as the matrix, making the endotoxin adsorbent prone to produce particles during hemoperfusion, which brings risks to the use

Method used

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  • Endotoxin absorbent and preparation thereof
  • Endotoxin absorbent and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A. Sodium periodate activated spherical porous cellulose

[0038] First vacuum filter the spherical porous cellulose to remove surface moisture.

[0039] Add 50 grams of spherical porous cellulose to the three-necked flask, then add 770 ml of purified water, control the temperature at 22 ± 1 ° C, stir to suspend the spherical porous cellulose in the purified water to form a spherical porous cellulose suspension.

[0040] Weigh 1.3 g of sodium periodate in a beaker, add 20 ml of water to dissolve, and make a sodium periodate solution. Add sodium periodate solution into the flask, then add 10ml of water to wash the beaker, pour the washing solution into the flask, continue stirring for 1 hour, raise the temperature to 31±1°C and stir for 10 minutes, filter out the reaction residue, and activate the cellulose The balls were washed twice with purified water and stored at room temperature.

[0041] B. Activated cellulose spheres immobilized polymyxin B

[0042] First weig...

Embodiment 2

[0047] A. Sodium periodate activated spherical porous cellulose

[0048] First vacuum filter the spherical porous cellulose to remove surface moisture.

[0049] Add 50 grams of spherical porous cellulose to the three-necked flask, then add 770 ml of purified water, control the temperature at 26 ± 1 ° C, stir to suspend the spherical porous cellulose in the purified water to form a spherical porous cellulose suspension.

[0050] Weigh 0.8 g of sodium periodate in a beaker, add 20 ml of water to dissolve, and make a sodium periodate solution. Add sodium periodate solution into the flask, then add 10ml of water to wash the beaker, pour the washing solution into the flask, continue to stir for 3 hours, raise the temperature to 34±1°C and stir for 20 minutes, filter out the reaction residue, and activate the cellulose The balls were washed twice with purified water and stored at room temperature.

[0051] B. Activated cellulose spheres immobilized polymyxin B

[0052] First weig...

Embodiment 3

[0057] A. Sodium periodate activated spherical porous cellulose

[0058] First vacuum filter the spherical porous cellulose to remove the surface moisture.

[0059] Add 50 grams of spherical porous cellulose to the three-necked flask, then add 770 ml of purified water, control the temperature at 16 ± 1 ° C, stir to suspend the spherical porous cellulose in the purified water to form a spherical porous cellulose suspension.

[0060] Weigh 1.6 g of sodium periodate in a beaker, add 20 ml of water to dissolve, and make a sodium periodate solution. Add sodium periodate solution into the flask, then add 10ml of water to wash the beaker, pour the washing liquid into the flask, continue to stir for 1 hour, raise the temperature to 31±1°C and stir for 30 minutes, filter out the reaction residue, and activate the cellulose The balls were washed twice with purified water and stored at room temperature.

[0061] B. Activated cellulose spheres immobilized polymyxin B

[0062] First wei...

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Abstract

The invention relates to an endotoxin adsorbent which is composed by taking spherical porous cellulose as a carrier and immobilizing effective amount of polymyxin B as a ligand. The preparation method of the endotoxin adsorbent includes that the spherical porous cellulose is taken as the carrier, the spherical porous cellulose first reacts with sodium periodate and is bonded with the effective amount of polymyxin B, and the endotoxin adsorbent is obtained after reduction by sodium borohydride. The endotoxin adsorbent has the advantages of stable performance, large supported quantity of polymyxin B, good adsorption effect, etc.

Description

technical field [0001] The invention relates to an endotoxin adsorbent and a preparation method thereof, in particular to an endotoxin adsorbent for hemoperfusion and a preparation method thereof. Background technique [0002] Endotoxin is a kind of lipopolysaccharide produced by cleavage of the cell wall when Gram-negative bacteria are released when they grow or die. A very small amount (nanogram level) of endotoxin entering the human body will cause high fever, dilation of blood vessels, and even fainting or death . Endotoxemia is one of the most common clinical fatal diseases, and the mortality rate can reach 40%-90%. [0003] There is no clinically effective method for treating endotoxemia, and one of the existing methods is hemoperfusion through an adsorbent bound to polymyxin (PMB). Among them, polymyxin is a cyclic decapeptide, which is the N-terminus of cyclic structure 7-peptide and 3-peptide, containing lipophobic and lipophilic groups and a polypeptide tail. Po...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30A61M1/34A61M1/00
Inventor 董凡
Owner JAFRON BIOMEDICAL
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