Modification method for animal skin collagen

Abstract

The invention discloses a method for modifying a collagen body of animal skin, which is characterized in that: 100 weight portions of collagen body material is taken and is added into 800 to 2,000 weight portions of 40 to 80v / v percent alcohol solution of 2-N-morpholino ethanesulfonic acid (MES) with a concentration between 20 and 70mmol / L and a pH value between 5.0 and 6.0 or a phosphate buffer solution (PBS) with the pH value between 5.0 and 6.0, the mixture is oscillated for 15 to 60min, then is respectively added with 2 to 30 weight portions of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS), is oscillated for 4 to 24h at room temperature to produce a collagen body modification material; or 100 weight portions of the collagen body material is taken, is added into 800 to 2,000 weight portions of the phosphate buffer solution (PBS) with the pH value between 6.0 and 8.0, is oscillated for 15 to 60min, then is added with 3 to 15 weight portions of genipin, and is oscillated for 10 to 24h at a temperature of between 20 and 40 DEG C to produce the collagen body modification material; then a monobasic sodium phosphate solution with the concentration of 0.1mol / L, the phosphate buffer solution with the pH value between 7.2 and 7.4 and double distilled water are orderly used to repeatedly clean the collagen body modification material, each cleaning time is not less than 60min, the cleaning time of each cleaning solution is not less than 3h, and the total cleaning time reaches more than 24h; and finally a modified collagen body material is produced by freeze drying.

Description

technical field [0001] The invention relates to a method for modifying animal skin collagen body with carbodiimide and genipal modifiers. Background technique [0002] Collagen from animal skin not only has unique physical and chemical properties, but also has excellent properties such as good biocompatibility, biodegradability, low immunity and hemostatic performance, especially it is easily absorbed in animals, and is friendly It is considered to be one of the most ideal biomedical materials because of its strong water properties, no toxic side effects, and good safety. Collagen derived from animal skin can be divided into two types, namely collagen bulk and hydrolyzed collagen. Collagen body refers to the animal tissue or organ with collagen as the main component, without or after a small amount of chemical or physical treatment, the animal tissue or organ that retains the structure and properties of natural collagen. [0003] At present, there are relatively few litera...

AI Technical Summary

Problems solved by technology

[0009] 1. The prior art uses chemical methods such as glutaraldehyde or physical methods such as irradiation and thermal dehydrogenation to modify collagen body materials used in the biomedical field, which has certain disadvantages such as cytotoxicity or difficulty in controlling the degree of crosslinking.

[0010] 2. In the prior art, the modification of tissue engineering scaffold materials by carbodiimide or genipin mainly focuses on natural polymer materials such as hydrolyzed collagen and chitosan, ignoring the feasibility and feasibility of applying them to collagen bulk materials. potential