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Long term storage process of biological sample

A biological sample and long-term preservation technology, applied in the field of molecular biology, can solve problems such as mildew or corruption, affect downstream inspection, biomolecular degradation, etc., achieve long-term preservation, avoid deliquescence and mildew, and slow down the speed of degradation and corruption Effect

Inactive Publication Date: 2008-11-19
长春市博坤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods have certain disadvantages, such as cryopreservation, which requires high cost and a lot of space to purchase and place low-temperature refrigerators, and even build cold storage, and once the power failure or instrument failure will easily lead to repeated freezing of samples. melted, or even corrupted, so that the biomolecules degrade and cannot be used for biological testing of samples
Although the cool and dry method is simple, due to the great differences in climate conditions in different places and in different months, the samples often cannot be kept in a cool and dry state, resulting in mildew or corruption, which affects downstream inspections. Therefore, the above method is suitable for most technologies and application sectors, it is difficult to achieve long-term storage of large quantities of biological samples

Method used

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  • Long term storage process of biological sample
  • Long term storage process of biological sample
  • Long term storage process of biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take 10ul of blood, smear it on the blood sample collection card or gauze produced by our company, and dry it in a cool place at room temperature to make a blood spot sample. Then put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 1000Pa, the relative humidity is 30%, and then seal it, and store the sealed blood spot sample stored in the vacuum bag in a cool place at room temperature . After being stored for 10 years, it was unpacked, and the DNA of the blood spot sample was extracted by the Chelex method, and the composite STR amplification test was performed. The results are shown in Figure 1.

Embodiment 2

[0013] Take a piece of costal cartilage, put it in a cool place to dry at room temperature, then put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 100Pa, and the relative humidity is 50%, then seal it, and store it in a vacuum bag Store the inner costal cartilage in a cool place at room temperature. After being preserved for 12 years, the package was unpacked, and the DNA of the costal cartilage was extracted by the Chelex method, and the multiple STR amplification test was carried out. The results are shown in Figure 2.

Embodiment 3

[0015] Take a dry saliva spot sample stored on filter paper, put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 500Pa, and the relative humidity is 10%, then seal it, and store it in a vacuum bag The saliva spot samples in the bag were stored in a cool place at room temperature. After being stored for 13 years, it was unpacked, the DNA of the saliva spot sample was extracted by the Chelex method, and the composite STR amplification test was performed. The results are shown in Figure 3.

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Abstract

The invention relates to a biological sample preservation method, which belongs to the technology field of the molecular biology. The invention aims to overcome the deficiency of the traditional biological sample preservation method, and provide a simple, convenient and novel biological sample preservation method. In the invention, through controlling the vacuum degree and the relative humidity in a vacuum bag, the dried biological samples preserved in the vacuum bag can be always maintained in the vacuum dried state. The biological samples dried in shade are placed into the vacuum bag, a drying agent is added, vacuumizing is performed, so that the vacuum degree is between 0.1 and 105Pa, and the relative humidity is between 1-50 percent, then sealing is performed, and the sealed biological samples preserved in the vacuum bag are placed in shade, and are preserved at normal temperature or low temperature for a long term. The biological sample preservation method can slow down the degeneration of the biological sample and the corruption speed, and avoid sample deliquescence and molding, and thereby the long-term preservation of the biological samples can be realized.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for preserving biological samples. Background technique [0002] For the field of molecular biology, especially for the field of DNA testing in criminal technology, it is always a difficult problem to find a low-cost method for long-term preservation of biological samples. At present, the commonly used biological sample preservation methods that do not use other reagents mainly include the following two methods: 1. Directly store biological samples at low temperature; 2. Adhere liquid biological samples such as blood and saliva to carriers such as filter paper, gauze, and collection cards , after drying in a cool and dry place, store at room temperature or low temperature. However, these two methods have certain disadvantages, such as cryopreservation, which requires high cost and a lot of space to purchase and place low-temperature refrigerators, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00A01N3/00
Inventor 杨百全王刚董涛胡海吴晓明
Owner 长春市博坤生物科技有限公司
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