Long term storage process of biological sample
A biological sample and long-term preservation technology, applied in the field of molecular biology, can solve problems such as mildew or corruption, affect downstream inspection, biomolecular degradation, etc., achieve long-term preservation, avoid deliquescence and mildew, and slow down the speed of degradation and corruption Effect
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Embodiment 1
[0011] Take 10ul of blood, smear it on the blood sample collection card or gauze produced by our company, and dry it in a cool place at room temperature to make a blood spot sample. Then put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 1000Pa, the relative humidity is 30%, and then seal it, and store the sealed blood spot sample stored in the vacuum bag in a cool place at room temperature . After being stored for 10 years, it was unpacked, and the DNA of the blood spot sample was extracted by the Chelex method, and the composite STR amplification test was performed. The results are shown in Figure 1.
Embodiment 2
[0013] Take a piece of costal cartilage, put it in a cool place to dry at room temperature, then put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 100Pa, and the relative humidity is 50%, then seal it, and store it in a vacuum bag Store the inner costal cartilage in a cool place at room temperature. After being preserved for 12 years, the package was unpacked, and the DNA of the costal cartilage was extracted by the Chelex method, and the multiple STR amplification test was carried out. The results are shown in Figure 2.
Embodiment 3
[0015] Take a dry saliva spot sample stored on filter paper, put it in a vacuum bag, put in a desiccant, vacuumize, the vacuum degree is 500Pa, and the relative humidity is 10%, then seal it, and store it in a vacuum bag The saliva spot samples in the bag were stored in a cool place at room temperature. After being stored for 13 years, it was unpacked, the DNA of the saliva spot sample was extracted by the Chelex method, and the composite STR amplification test was performed. The results are shown in Figure 3.
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