Preparation method for enriching gamma-aminobutyric acid by using fixed rice bran glutamic acid decarboxylase

Abstract

The invention relates to a preparation method of enriching GABA by immobilized rice bran glutamate decarboxylase, and belongs to the technology field of the deep processing of the rice bran. In the method, the rice bran which is byproducts obtained after paddies are processed is used as raw material; the rice bran is mixed with sodium alginate solution with certain concentration and evenly stirred after being crushed and screened, and then is put in a balling device; CaCl2 solution is dropwise dropped into the mixed solution to act spherulization; ball bodies are sclerotized; the redundant CaCl2 and the rice bran which is not immobilized are washed, so that the immobilized rice bran glutamate decarboxylase is obtained; the immobilized rice bran glutamate decarboxylase is mixed with PBS buffer solution, sodium glutamate is added in and used as zymolyte, the concentration of the sodium glutamate is 0.01 to 0.05mol / l, the mixed solution reacts in a table concentrator under the temperature of 45 DEG C to obtain GABA enriched solution, and products are obtained after being dried. The protein content and the sugar content thereof are only a tenth part of the products which are obtained by adopting a traditional method of enriching the GABA by directly utilizing the rice bran; the products obtained by the method can be used as functional food additives; the efficacies of lowering blood pressure, calming, aiding sleeping, protecting liver, etc. can be realized; the preparation method has scientific and reasonable design, and has great significance to realize the comprehensive utilization of rice bran resources and increase the added value thereof.

Description

technical field [0001] The invention discloses a preparation method for enriching gamma-aminobutyric acid (GABA) by immobilized rice bran glutamic acid decarboxylase, which belongs to the technical field of rice bran deep processing. Background technique [0002] Rice is one of the main food crops on the earth. China has always been known as the "Kingdom of Rice". The annual rice output accounts for about 35% of the world's total rice output, ranking first in the world. As a by-product of rice, rice bran has a very rich source, and if it is well utilized, it can achieve high added value. The activity of glutamate decarboxylase in rice bran is very high, which can be used as a catalyst to catalyze the decarboxylation reaction of monosodium glutamate to generate GABA. However, the vast majority of rice bran resources in my country are consumed in the form of traditional feed, which is very wasteful. [0003] Various foods with high GABA content are now sold in the market in ...

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Problems solved by technology

However, most of the rice bran resources in my country are consumed in the form of traditional feed, which is very wasteful.

[0003] Various foods with high GABA content are now sold in the market in Japan, such as germinated brown rice, GABA tea, GABA skin care products, health care products, etc., and the market is very good, but there are no such products on the market in my country, and we are urgently waiting for Chinese manufacturers Supplement the domestic blank

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