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Industrial culture method for liquorice cell

A culture method and cell technology, applied in the direction of plant cells, etc., can solve the problems of systematic research on licorice cell suspension culture and industrial culture, and few researches on licorice cell culture. The effect of fast growth of injured tissue and stable yield

Inactive Publication Date: 2008-09-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the reports on tissue culture of Glycyrrhiza mainly focus on the research of callus culture, differentiation and hairy root culture, while the research on Glycyrrhizae cell culture is less; at the same time, the systematic research and industrialization of Glycyrrhizae cell suspension culture Cultivation has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Soak 3 g of licorice seeds (purchased from Beijing Shizhen Institute of Chinese Herbal Medicine) with a mass concentration of 70% ethanol for 10 to 15 seconds, rinse twice with sterilized deionized water, and then use a mass concentration of 2% sodium hypochlorite solution Soak for 15 minutes, then rinse twice with sterilized deionized water, inoculate it in 20mL MS medium for solid culture, the culture conditions are: temperature 23°C, light intensity 800lux, light time 12h / d, culture about 3d seeds give birth to buds, and cut the buds into small pieces of 3-4 mm. Take 25mL of MS medium, add 0.025mg of 2,4-D therein, add 0.05mg of KT, add 0.75g of sucrose, add 0.25g of agar, make a callus culture medium, use mass concentration as 10% HCl adjustment The pH is 4.5. Place the small bud segments in the above-mentioned cultivation for cultivation. The culture conditions are as follows: the temperature is 23°C, culture is protected from light, and after 20 days of culture,...

example 2

[0017] Soak 4 g of licorice seeds with 70% ethanol for 10 to 15 seconds, rinse them twice with sterilized deionized water, soak them with 2% sodium hypochlorite solution for 16 minutes, and then sterilize them with deionized water. Rinse twice with deionized water, inoculate it in 20mL MS medium for solid culture, the culture conditions are: temperature 25°C, light intensity 1000lux, light time 12h / d, after culturing for about 3 days, the seeds sprouted, and the buds were cut into 3 Small segments of ~4 mm. Take 25mL of MS medium, add 0.05mg of 2,4-D, 0.0125mg of KT, 0.75g of sucrose, and 0.25g of agar to make a callus culture medium, and adjust it with NaOH with a concentration of 1mol / L. The pH is 5.8. Place the small bud segments in the above-mentioned cultivation for cultivation. The culture conditions are as follows: the temperature is 25°C, culture is protected from light, and after 20 days of culture, the culture dish is covered with licorice callus.

[0018] Transfe...

example 3

[0021] Soak 5 g of licorice seeds in 75% ethanol for 10 to 15 seconds, rinse them twice with sterilized deionized water, soak them in 2% sodium hypochlorite solution for 20 minutes, and then sterilize them with deionized water. Rinse it twice with deionized water, inoculate it in 20mL MS medium for solid culture, the culture conditions are: temperature 26°C, light intensity 1500lux, light time 12h / d, after culturing for about 3 days, the seeds sprouted, and the buds were cut into 3 Small segments of ~4 mm. Take 25mL of MS medium, add 0.1mg of 2,4-D, 0.0025mg of KT, 0.75g of sucrose, and 0.25g of agar to make a callus culture medium, and adjust it with NaOH with a concentration of 1mol / L. The pH is 6.5. Place the small bud segments in the above-mentioned cultivation for cultivation. The culture conditions are as follows: the temperature is 25°C, culture is protected from light, and after 20 days of culture, the culture dish is covered with licorice callus.

[0022] 6g callus...

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PUM

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Abstract

The invention discloses a method for culturing industrial liquorice cells, and belongs to the liquorice cell culture technology. The method comprises the steps of inoculating liquorice seeds in an MS culture medium after sterilization for inducing aseptic sprouts of the liquorice; transferring cut liquorice sprout segments to a callus culture medium for solid culture so as to generate a liquorice callus; transferring the callus to a cell culture medium for suspension culture and then subculture so as to obtain liquorice cell sap with stable cell output; and transferring the liquorice cell sap to a stirring paddle type reactor for scale suspension culture of the liquorice cells to obtain liquorice cell suspension with cell output of 15-40g / l by cell dry weight. The method is characterized by simple process, quick cell growth, stable output, etc.

Description

technical field [0001] The invention relates to a suspension culture method of licorice cells, which belongs to the technology of licorice cell culture. Background technique [0002] Licorice (Glycyrrhiza uralensis Fisch.) is a perennial herb of the leguminous family. Its roots and rhizomes have the functions of invigorating the spleen and Qi, relieving cough and expectorant, clearing away heat and detoxification, and harmonizing various medicines. It is one of the most commonly used traditional Chinese medicines. Fang Jiucao" said. Modern pharmacological studies have shown that licorice has a wide range of pharmacological activities, such as detoxification, anti-oxidation, and anti-inflammation. The main active ingredients in licorice are flavonoids and triterpenoids. Its main triterpenoid component, glycyrrhizic acid, has various pharmacological activities such as anti-inflammation, anti-ulcer, anti-virus, and immune regulation. It has also been reported that glycyrrhizi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 高文远卞爱华王娟曹宇
Owner TIANJIN UNIV
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