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Method for separating catalpol from fresh rehmanniae root

A kind of technology of fresh Rehmannia glutinosa, catalpol, applied in directions such as chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of reducing production costs

Inactive Publication Date: 2008-09-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is currently no report of catalpol with kilogram-level purity higher than 98% provided by any manufacturer or unit

Method used

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  • Method for separating catalpol from fresh rehmanniae root
  • Method for separating catalpol from fresh rehmanniae root
  • Method for separating catalpol from fresh rehmanniae root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1) Shredded and homogenized fresh rehmannia glutinosa

[0053] Take fresh rehmannia glutinosa, discard the rotten parts, wash, peel (except for the white and light yellow chrysanthemum cores in the middle of fresh rehmannia glutinosa), and cut into slices with a thickness of 1mm to 2cm;

[0054] Under anaerobic conditions, immediately heat fresh rehmannia slices to 80°C-100°C, keep warm for 70min-80min; or directly heat-treat with water vapor for 60min-80min; then homogenize at 6000rpm for 10min;

[0055] Or, immediately mix fresh rehmannia slices with its equal weight of hot water at 100°C, and homogenize together at 5000 rpm for 20 minutes;

[0056] Routinely filter the homogenate to remove unbroken fresh rehmannia tissue and insoluble impurities to obtain a sample solution containing catalpol.

[0057] 2) Macroporous adsorption resin separation

[0058] Take about 5kg of macroporous adsorption resin HPD-100 and wet-pack the column with a column volume of about 5000...

Embodiment 2

[0068] 1) Shredded and homogenized fresh rehmannia glutinosa

[0069] Take fresh rehmannia glutinosa, discard the rotten parts, wash, peel (except for the white and light yellow chrysanthemum cores in the middle of fresh rehmannia glutinosa), and cut into slices with a thickness of 1mm to 2cm;

[0070] Immediately heat the fresh rehmannia slices to 40°C-50°C, keep warm for 100min-120min; or directly heat-treat with water vapor for 90min; then homogenize at 5000rpm for 15min;

[0071] Alternatively, immediately mix fresh rehmannia slices with its equal weight of hot water at 60°C, and homogenize together at 5000 rpm for 20 minutes;

[0072] Routinely filter the homogenate to remove unbroken fresh rehmannia tissue and insoluble impurities to obtain a sample solution containing catalpol.

[0073] 2) Macroporous adsorption resin separation

[0074] Take about 5kg of macroporous adsorption resin HPD-100 and wet-pack the column with a column volume of about 5000mL. The sample sol...

Embodiment 3

[0082] 1) Shredded and homogenized fresh rehmannia glutinosa

[0083] Take fresh rehmannia glutinosa, discard the rotten parts, wash, peel (except for the white and light yellow chrysanthemum cores in the middle of fresh rehmannia glutinosa), and cut into slices with a thickness of 1mm to 2cm;

[0084] Immediately heat the fresh rehmannia slices to 100°C and keep warm for 20 minutes; or directly heat-treat with water vapor for 30 minutes; then homogenize at 8000rpm for 5 minutes;

[0085] Alternatively, immediately mix fresh rehmannia slices with its equal weight of hot water at 80°C, and homogenize together at 6000 rpm for 20 minutes;

[0086] Routinely filter the homogenate to remove unbroken fresh rehmannia tissue and insoluble impurities to obtain a sample solution containing catalpol.

[0087] 2) Macroporous adsorption resin separation

[0088] Take about 5kg of macroporous adsorption resin HPD-100 and wet-pack the column with a column volume of about 5000mL. The sampl...

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Abstract

The invention discloses a method for separating catalpol from fresh rehmannia root, which includes pulverizing and homogenizing fresh rehmannia root to obtain catalpol-containing sample solution; separating macroporous absorption resin; and performing chromatography with a silica gel column or re-crystallizing. The method maximally separates the catalpol from saccharide component in the rehmannia root to clear away obstacles for the preparation of high purity catalpol. The method has high production capability of the high purity catalpol of up to 100 kg catalpol / year, energy saving effect, environmental friendliness, and low production cost, and provides raw material guarantee for research, drug development, and health product development.

Description

technical field [0001] The invention relates to a method for separating catalpol, in particular to a method for separating catalpol from fresh rehmannia glutinosa. Background technique [0002] Rehmannia glutinosa is a commonly used bulk Chinese herbal medicine, with a demand of about 20,000 tons / year and an export of about 1,000 tons / year. Due to the difference in medicinal efficacy, it can be used as different medicinal materials. It is a typical medicinal material whose medicinal properties have been changed after processing; it is divided into fresh rehmannia glutinosa, raw rehmannia glutinosa and rehmannia glutinosa. Fresh rehmannia clears away heat and promotes body fluid, cools blood to stop bleeding, and is used for febrile disease and yin deficiency, and red tongue and polydipsia; raw rehmannia root clears heat and cools blood, nourishes yin and promotes body fluid, and is used for red tongue and polydipsia, yin deficiency and internal heat; rehmannia glutinosa nour...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K36/804
Inventor 温学森赵宇刘彦飞
Owner SHANDONG UNIV
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