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Y gene order of benzene induced aplastic anemia related antigen specific TCR V alpha 19

An aplastic and gene sequence technology, applied in the field of nucleotide sequences of T cell receptors, can solve the problem of lack of research data

Inactive Publication Date: 2008-08-27
JINAN UNIVERSITY
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Problems solved by technology

However, there are few research data on the TCRα subfamily and its complementarity-determining region 3 (CDR3), that is, the site that specifically recognizes and binds to antigens, in occupational benzene-injected populations

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  • Y gene order of benzene induced aplastic anemia related antigen specific TCR V alpha 19

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Embodiment

[0033] (1) Analysis of antigen-specific TCR gene sequence

[0034] There are many methods for analyzing the length and base sequence of CDR3 of TCRVα gene spectrum, and the commonly used method is to use RT-PCR-gene scanning method to analyze the length of CDR3 of 29 subfamilies of TCRVα. First, the peripheral blood of patients with aplastic anemia caused by benzene was collected, and the peripheral blood mononuclear cells were separated by Ficoll layering method, and 1*10 7RNA was extracted from PBMCs (peripheral blood mononuclear cells), and cDNA was synthesized by RT-PCR. At the same time, the gene sequence of the TCRα chain was found in the NCBI gene bank, and the positions of the V and C regions of each subfamily in the genome were marked. Design corresponding 29 Vα-specific upstream primers based on the cDNA of 29 Vα subfamily genes, and set a Cα downstream primer in the Cα region, and use PCR technology to amplify the V-N-JC region generated by the TCR rearrangement of...

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Abstract

The invention discloses a nucleotide sequence of a specificity T cell receptor aiming at associated antigens of aplastic anemia by benzene. RT-PCR gene scanning analysis discovers that TCR V alpha 19 subfamily appears obvious monoclone in peripheral blood of patients of aplastic anemia by benzene, a V-N-J region of a specificity TCR sequence is discovered through analyzing a sequence of a PCR product, the V-N-J region is a complementarity determining region and a part which is combined with antigen specific recognition, which shows that TCR of clonal T cells is specificity PCR which is produced through stimulating the associated antigens of aplastic anemia by benzene. The nucleotide sequence is the basis and the key for immune targeted therapy of the aplastic anemia by benzene. The nucleotide sequence of the specificity T provides conditions for further developing cellular immunotherapy of the aplastic anemia by benzene.

Description

technical field [0001] The invention relates to a T cell receptor (TCR) nucleotide sequence, in particular to a Y gene sequence of a benzene-induced aplastic anemia (AA) related antigen-specific TCRVα19 subfamily. Background technique [0002] Benzene is one of the most widely used chemical raw materials and industrial solvents. Occupational benzene workers in my country have reached more than 500,000, accounting for the first in the number of occupational exposure to various chemicals. Both the International Agency for Research on Cancer (IARC) and the US Environmental Protection Agency (EPA) list benzene as a confirmed human carcinogen. A cohort study conducted by Chinese scholars and the National Cancer Institute (NCI) on 74,828 benzene-injected workers and 3,580 control workers in China proved that benzene-injected workers developed malignant tumors of the blood and lymphatic system, such as aplastic anemia (AA), bone marrow The relative risk of dysplastic syndrome (MD...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/10C07K14/725
Inventor 李扬秋李萡陈少华杨力建
Owner JINAN UNIVERSITY
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