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A culture method for Trichoderma reesei that improves cellulase production

A technology of Trichoderma reesei and cellulase, applied in the field of microbial cultivation in biochemistry

Active Publication Date: 2012-02-01
CHINA PETROLEUM & CHEM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after exceeding a certain limit value, dissolved oxygen is also toxic and inhibitory to microbial growth

Method used

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  • A culture method for Trichoderma reesei that improves cellulase production
  • A culture method for Trichoderma reesei that improves cellulase production
  • A culture method for Trichoderma reesei that improves cellulase production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Trichoderma reesei mass-produces cellulase by fixed ventilation

[0024] Trichoderma reesei liquid activation medium is: 6g glucose, 0.6g peptone, 0.6mL Mandels trace elements, 60mL Mandels nutrient salt, 30mL citric acid buffer solution with a concentration of 1mol / L, add 510mL distilled water, and pack in conical Sterilized in bottles.

[0025] Trichoderma reesei enzyme production medium is: glucose 6g, sulfate pulp 60g (dry weight), ammonium sulfate 13.2g, urea 3g, potassium dihydrogen phosphate 12g, calcium chloride 1.8g, magnesium sulfate 1.8g, 6mL of Mandels For trace elements, add water until the total liquid volume is 6L, and heat up to 100°C for sterilization.

[0026] Put the Trichoderma reesei species into 50mL liquid activation medium, culture it in a constant temperature oscillator at 26-32°C and 150-250r / min for 36h, put it into a 10L fermenter at a ratio of 1:10, fill it with a liquid volume of 6L, and ventilate Cultivate and prepare cellu...

Embodiment 2

[0030] Embodiment 2: Trichoderma reesei regulates ventilation and mass-produces cellulase

[0031] Except ventilation rate, other conditions are the same as embodiment 1. The initial ventilation rate is controlled at 10vvh, and the change of the dissolved oxygen concentration is monitored. When the dissolved oxygen concentration drops to 20%, the ventilation rate is increased, and when the dissolved oxygen concentration rises to 30%, the ventilation rate is reduced. The concentration of dissolved oxygen will rise in the late stage of enzyme production, so the ventilation rate should be reduced until it drops to 10vvh.

[0032] The concentrations of oxygen and carbon dioxide in the tail gas measured during the enzyme production process are as follows: image 3 shown. The highest concentration of carbon dioxide in the tail gas is about 0.4%, which is only half of that of Example 1, which is beneficial to the growth and metabolism of mycelia.

[0033] The adjustment of the ven...

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PUM

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Abstract

A method for increasing the productivity of Trichoderma reesei (Trichodermareesei) cellulase by adjusting the ventilation volume to control the dissolved oxygen concentration of the solution. It is characterized in that the ventilation volume is adjusted during the enzyme production process to control the dissolved oxygen concentration in the main stage of enzyme production at 20% to 30%. This not only ensures that the dissolved oxygen concentration of the enzyme production liquid is not lower than the critical concentration, but also reduces the carbon dioxide concentration in the exhaust gas. Conducive to bacterial growth. Using this method to produce enzymes, compared with mass-produced enzymes with fixed ventilation, the enzyme activity of filter paper can be increased by 30% and the enzyme production time can be shortened by 14%.

Description

1. Technical field [0001] The invention belongs to the technical field of microbial cultivation in biochemistry, and in particular relates to a method for cultivating Trichoderma reesei for improving the production of cellulase. 2. Background technology [0002] As the world's oil reserves are increasingly depleted, ethanol has become one of the most important liquid fuel alternatives. Vigorously developing new renewable energy sources is a prerequisite for China's sustainable development in the 21st century. Ethanol is a renewable energy produced through sugar fermentation. Traditional ethanol fermentation uses grain as raw material, but it is becoming increasingly unrealistic to rely on grain to produce fuel ethanol for a long time. The most abundant renewable resource on earth - plant cellulose It will be the best raw material for ethanol production in the future. Cellulose is a linear polymer connected by β-1,4-glucosidic bonds. Its degradation into monosaccharides is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/885C12N1/14C12N9/42
Inventor 余世袁勇强杭志喜
Owner CHINA PETROLEUM & CHEM CORP
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