High content and high activity oral polysaccharide-peptide and preparing method and application of the same

Abstract

The invention relates to a polysaccharide-peptide with high content and high activity, a preparation method thereof and an application thereof. The polysaccharide-peptide is freely soluble in water; the total amount of glycopeptides is equal to or more than 70 percent; the polysaccharide-peptide with the average weight over 10kd takes up more than 80 percent of the total amount of glycopeptides, wherein, the sugar content is 50 to 80 percent, and the peptide content is 16 to 30 percent; the preparation method is as follows: (1) manyzoned polypore crude extract is made available to be suspended or dissolved in the water at the temperature of 80 to 100 DEG C; the manyzoned polypore crude extract solution is refined and separated, or the hot water with the temperature of 80 to 100 DEG C is used for extracting polyporus versicolor mycelium or manyzoned polypore fruit body for producing the manyzoned polypore crude extract solution; (2) the pH value of the manyzoned polypore crude extract solution or the manyzoned polypore crude extract suspended solution is controlled to be between 4 to 8; the lauter extracting solution can be obtained after filtering or centrifugal separation, which is concentrated to the specific gravity of 1.05 to 1.3; (3) a decoction and alcohol sedimentation method is used for purification. Compared with prior polysaccharide-peptide, the product obtained has more effective anti-tumor effect and better protective effect on marrow function and immune function, which are suitable for preventing and treating tumors.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to an orally absorbed high-content and high-activity versicolor glycopeptide, a preparation method and application thereof. Background technique [0002] Yunzhi glycopeptide is a crude glycopeptide extracted from Yunzhi submerged mycelium by water extraction and alcohol precipitation. It is one of the few glycopeptide immune enhancers that can be orally absorbed. It can inhibit the proliferation of various cancer cells such as human lung cancer, gastric cancer, esophageal cancer, leukemia, ascites cancer, etc. At the same time, it can effectively improve human immune function, increase appetite, relieve pain, improve hypoxia resistance, and reduce toxic and side effects caused by radiotherapy and chemotherapy , has been marketed as an adjuvant therapy drug for tumors in China. Chinese patent 89105471.5 reports "a production method of versicolor glycopeptide", the extraction met...

AI Technical Summary

Problems solved by technology

However, its product and its purification process still have three deficiencies: First, although the free protein can be removed by using the Savege method for deproteinization, it inevitably removes some polysaccharide peptides and polysaccharides with physiological activity, resulting in active ingredients Second, the use of ultrafiltration membranes with a molecular weight of 30Kd and 300Kd to cut off and remove substances with a molecular weight less than 30Kd and greater than 300Kd also caused a corresponding loss of active glycopeptides, and the product yield further decreased

Third, after multiple purifications, although the polysaccharide content in the product has increased from 35-40% to more than 75%, the product yield has doubled and the cost has doubled, so it can only be used as a raw material for traditional Chinese medicine for injection, and its Glycopeptides, especially glycopeptide traditional Chinese medicine injections containing some free proteins and a large amount of unknown impurities, inevitably produce allergic reactions and other toxic and side effects during injection administration, which increases the clinical risk of tumor patients