Multi-PCR detection method for minim DNA

Abstract

The invention relates to a multi-PCR detection method of trace DNA, which can avoid the competition inhibition problem due to the excessive primers of the multi-PCR technology by changing the concentration and the adding mode of the primers, so the multi-PCR detection method can be used for the very limited DNA of an initial sample, in particular to a plurality of very valuable clinical samples, such as, fertilized eggs and the obtained cells by laser capture and microdissection, etc. The whole detection proposal of the invention has simple operation process, high specificity, very high sensitivity and PCR efficiency, easy automation and wide application prospect in practical application.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection, and in particular relates to a multiplex PCR technology which realizes supersensitive detection of trace DNA by changing the concentration of primers and the way of adding primers. Background technique [0002] Polymerase chain reaction (PCR) is the most commonly used amplification technology in biological detection today, and it can amplify a certain DNA fragment hundreds of thousands to millions of times within a few hours. And with the popularization and application of fluorescent quantitative PCR, the quantification of the target sequence can also be completed accurately. However, in general PCR, only one pair of primers is used, the throughput is low, and the initial template concentration is required, so the sensitivity is low. But for many studies, the DNA of the starting sample is very limited, especially some very precious clinical samples, such as fertilized eggs, cells obtained b...

AI Technical Summary

Problems solved by technology

The whole operation is simple and easy, and can effectively detect trace amount of template DNA, but this method is limited to single-site analysis and cannot perform multi-site analysis

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