Core-peptide composition with physiological activity and its preparation method and application
A peptide complex, physiologically active technology, applied in the field of biomedicine, to achieve the effect of remarkable purification effect, easy operation and improving the level of free testosterone
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Embodiment 1
[0048] Take 100g of red snail tissue (trunk, liver, reproductive organs) and mash it in a frozen state, add 10 times the amount of 95% ethanol to extract for 1 hour, filter, then stir and extract with 50% acidic ethanol for 3 hours, filter, combine the two extracts, Let cool, precipitate out, pour out the supernatant, concentrate, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40 ℃, add vitamin E to make the final concentration to 0.03%, concentrate under reduced pressure to remove the solvent, obtain the crude product, and then use sephadex LH20 column chromatography to separate, the volume ratio is 5: 3 methanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) ) to elute the nucleopeptide complex.
[0049] After chemical analysis and detection, the obtained nucleopeptide complex components include per m...
Embodiment 2
[0051] Take 100g of the whole tissue of the red snail and mash it in the frozen state, add 10 times the amount of 50% ethanol, adjust the pH value to not less than 4.5 with acetic acid, extract it in a container with mixing and stirring function for 2 hours, filter, concentrate, and let it cool The precipitate was precipitated, the supernatant was poured out, treated with 3 times the amount of hexane, the hexane layer was discarded, the lower lipoprotein layer was purified with 2% activated carbon, ultrafiltered, concentrated, the operating temperature was always kept below 40°C, and 0.02% Vitamin E was concentrated under reduced pressure to remove the solvent to obtain a crude product, which was then separated by sephadex LH20 column chromatography and eluted with a methanol-water composite solvent with a volume ratio of 5:2 to obtain a nucleopeptide complex.
[0052] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...
Embodiment 3
[0054] Take 100g of snail tissues (trunk, liver, reproductive organs) and crush them in a frozen state, add 8 times the amount of 80% ethanol, adjust the pH value to not less than 4.5 with acetic acid, stir and extract for 3 hours, filter, and concentrate to 1 / 2 of the original volume 10. Let the precipitate cool down, pour out the supernatant, treat with 3 times the amount of hexane, discard the hexane layer, purify the lower lipoprotein layer with 2% activated carbon, ultrafilter, concentrate, and keep the operating temperature below 40°C. , add 0.03% vitamin E, concentrate under reduced pressure to remove the solvent to obtain the crude product, then use sephadex LH20 column chromatography to separate, the volume ratio is 5:4 ethanol-water composite solvent (use acetic acid to adjust the eluent to be acidic) and elute to obtain the nucleus peptide complex.
[0055] After chemical analysis and detection, the obtained nucleopeptide complex components include per milliliter: 8...
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