Method for horizontal transfection of exogenesis endosymbiosis bacterium to bemisia tabaci gennadius
A technology of bemisia tabaci and symbiotic bacteria, applied in the field of agricultural biology, can solve the problems of poor fixation and easy death of bemisia tabaci adults, and achieve the effect of simple method, good technical platform and increased infection rate
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[0026] A method for horizontal transfection of exogenous endosymbiotic bacteria to whitefly whitefly, comprising preparation of exogenous endosymbiotic bacteria Wolbachia (Wolbachia), preparation of whitefly pseudopupal receptors, injection, and injection of whitefly whitefly For the cultivation of pseudopupal receptors, the specific steps are as follows:
[0027] With the eggs of the rice moth as the donor of the symbiotic bacteria Wolbachia (Wolbachia), 30 μL of the eggs of the rice moth were placed in a 200 uL sterilized tube, and 100 uL of SPG buffer (218 mM sucrose, 3.8 mM KH 2 PO 4 , 7.2 mM K 2 HPO 4 , 4.9mM L-glutamic acid, pH7.2) rinsed 4 times; In a 0.2mL sterile tube, use 100uL of SPG buffer to homogenize the eggs, centrifuge at 300g for 5min, and transfer the supernatant to a new tube ( Centrifuge at 12000g for 10min in 0.2mL), remove the supernatant after centrifugation, and resuspend the pellet with SPG buffer;
[0028] Centrifuge the suspension at 300g for 5 ...
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