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Application of PDCD4 recombined expression vector in preparing medicament for treating gonad cancer

An expression vector, ovarian cancer technology, applied in gene therapy, pharmaceutical formulations, genetic material components, etc., can solve the problems of deletion, low expression, lack of expression, etc., and achieve the effect of prolonging survival time, improving prognosis, and improving survival rate

Inactive Publication Date: 2010-09-08
SHANDONG UNIV
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Problems solved by technology

[0009] Similar to other tumor suppressor genes, PDCD4 has obvious expression loss in some human tumor cell lines and tumor specimens: Jansen AP et al. detected 60 tumor cells from kidney, lung, colon, breast and glial cells The expression of PDCD4 in the line showed that the expression of PDCD4 was low or absent at both the mRNA and protein levels, but the loss rate of PDCD4 at the protein level was significantly higher than that at the RNA level, confirming that human PDCD4 is also a tumor suppressor gene

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  • Application of PDCD4 recombined expression vector in preparing medicament for treating gonad cancer
  • Application of PDCD4 recombined expression vector in preparing medicament for treating gonad cancer
  • Application of PDCD4 recombined expression vector in preparing medicament for treating gonad cancer

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Embodiment Construction

[0037] The recombinant expression vector preferably used in the test of the present invention is a viral vector (purchased from Wuhan University Institute of Virology), and preferably used in the viral vector is an adenovirus:

[0038] 1. Construction and Production of PDCD4 Recombinant Adenovirus

[0039] (1) Construction of adenovirus expression vector

[0040] 1) Cloning of PDCD4 gene into pMD-18T plasmid: Design a pair of specific oligonucleotide primers according to the cDNA sequence of PDCD4 (including signal peptide). The upstream primer sequence is: 5'-CTGAGCACATATGATGAATCAAACTGCGATTCTGAT-3', and the downstream primer sequence is: 5'-GAGGATCCTTAAGGAGATCTTTTTAGACATTTC-3'. Using the phage human lung cDNA library as a template, using the above-mentioned upstream and downstream primers, the target gene PDCD4 was amplified with Pyrobest high-fidelity DNA polymerase. The PCR parameters are 94°C, 30s; 53°C, 1min; 72°C, 1min, 30 cycles. The PCR products were recovered by 1%...

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Abstract

The invention discloses an application of a PDCD4 recombinant expression vector on the preparation of a drug for ovarian cancer. Nucleotide sequence for coding a PDCD4 gene is inserted to the genome of the recombinant expression vector to prepare a chemical drug for ovarian cancer, which leads to the high level expression of the drug in tumor cells, the treatment of the drug for ovarian cancer and the restraint of the drug for cell growth and transfer. The invention provides strong experimental basis for the development of anticancer drugs and has great theoretical value as well as a good application prospect.

Description

technical field [0001] The invention relates to the application of a PDCD4 recombinant expression vector, in particular to the application of a PDCD4 recombinant expression vector in the preparation of drugs for treating ovarian cancer. Background technique [0002] Ovarian cancer is one of the three most common gynecological malignancies, and its mortality rate ranks first among gynecological malignancies. At present, surgical resection and combined chemotherapy are mainly used clinically, but the overall curative effect is not satisfactory. This is because ovarian cancer lacks a complete early diagnosis method, and 70% of the patients are already at an advanced stage when they see a doctor and cannot be operated on; in addition, most of the traditional anticancer drugs currently used in clinical practice are cytotoxic drugs, and their clinical efficacy is low. Often depends on the sensitivity of the cancer cells to the drug. Traditional anticancer drugs have relatively n...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00
Inventor 张利宁王晓燕高飞张霞魏增涛王群朱法良郭春
Owner SHANDONG UNIV
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