Nucleic acid extraction device

Abstract

The invention relates to a nucleic acid extracting device, which is characterized in that the nucleic acid extracting device comprises an oscillator component, a sample holder component, a control system and a frame; the oscillator component comprises an electric motor, an eccentric sleeve connected on the output shaft of the electric motor, a bearing connected on the eccentric shaft of the eccentric sleeve and a bearing sleeve connected on the external ring of the bearing; a threaded sleeve integrated with the bearing sleeve is arranged at the top of the bearing sleeve and a rotation stopping sleeve is arranged on the retaining plate of the electric motor; a plurality of extension springs are connected between the rotation stopping sleeve and the bearing sleeve; the sample holder component comprises a test tube stand, a plurality of sample tubes inserted and arranged on the test tube stand and a scotch yoke arranged at the top of the sample tube; the top of the scotch yoke is connected with a locking cover and the top of the locking cover is connected with a locking handle; the bottom screw rod of the locking handle passes through the locking cover, the scotch yoke and the test tube stand so as to be fixed in the threaded sleeve of the oscillator component and the scotch yoke is elastically connected with the test tube stand. The invention can be used for extracting the nucleic acids of the microorganicsms such as the bacteria (G+ bacteria, G-bacteria, mycobacterium), fungi (yeast, white candida), viruses, etc., including the extraction of the RNA.

Description

technical field [0001] The invention relates to a nucleic acid extraction device, in particular to a nucleic acid extraction device which realizes sample lysis by combining a certain reagent in a physical vibration manner. Background technique [0002] Life science is an experimental science. A large number of biological experiments and clinical diagnosis are inseparable from sample preparation. Nucleic acid extraction is widely used in sample preparation. With the development of biotechnology, the number of samples processed in clinical diagnosis and biological experiments is often very large. The traditional nucleic acid preparation method is to add the sample to be tested into a reagent containing a chemical reagent or lyase, and achieve the preparation of the nucleic acid sample through a chemical reaction. This method has many disadvantages such as toxic substances, time-consuming sample preparation, low efficiency, few samples to be processed, and cross-contamination....

AI Technical Summary

Problems solved by technology

This method has many disadvantages such as toxic substances, time-consuming sample preparation, low efficiency, few samples to be processed, and cross-contamination. It is gradually difficult to meet the requirements of high-speed and large-volume nucleic acid extraction.

Existing nucleic acid extractors can process a small number of samples at a time, the system structure is complex, the price is expensive, the extraction takes a long time, and often requires cumbersome steps such as adsorption, washing, and elution

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