Method for producing dry bacterium powder containing gamma-linolenic acid

A production method, the technology of linolenic acid, is applied in the field of improvement of the process of producing dry bacterial powder containing γ-linolenic acid, which can solve the problems of affecting product quality and poor survival rate of strains, and achieve stable product quality, short production cycle, and guaranteed The effect of product quality

Inactive Publication Date: 2008-05-28
崔亦鸿 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem is that at this temperature, the survival rate of the bacteria is poor, especially the γ-linolenic acid is prone to deterioration, which affects the quality of the final product

Method used

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  • Method for producing dry bacterium powder containing gamma-linolenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Use the γ-linolenic acid strains that exist in some spore plants and seed vegetable oils as culture and fermentation strains:

[0037] Screen the strains in the incubator;

[0038] The slant culture of the strain is to vibrate for 5 days at 25°C;

[0039] Fermented for 3 days at 25℃ in the first-level fermentation tank;

[0040] Fermentation for 4 days in a secondary fermentation tank at 25°C:

[0041] Fermented in a tertiary fermentation tank at 25°C for 3 days:

[0042] Fermented in a four-stage fermentation tank at 25°C for 4 days;

[0043] Then, the cells are filtered and dehydrated, and then dried under vibration at 25°C and 0.5 Pascal conditions or blow-dried in a vibration mode using air with a humidity of 20%-30% at -30°C.

[0044] Finally, it is crushed and sieved with an average particle size of 113 microns, and the products are aseptically vacuum packaged and stored in storage.

[0045] The above-mentioned slant culture means that the expanded cultured strains ar...

Embodiment 2

[0058] Use the γ-linolenic acid strains that exist in some spore plants and seed vegetable oils as culture and fermentation strains:

[0059] Screen the strains in the incubator;

[0060] The slant culture of strains is cultured at 28°C with shaking for 3 days;

[0061] Fermented for 3 days at 28℃ in the first-level fermentation tank;

[0062] Fermented for 3 days at 28°C in the secondary fermentation tank:

[0063] Fermented in a tertiary fermentation tank at 28°C for 3 days;

[0064] Fermented in a four-stage fermentation tank at 28°C for 4 days;

[0065] Vibrate drying at 25°C and 0.6 Pascal, or blow dry with air with a humidity of 25% at -30°C in a vibration mode.

[0066] Finally, it is crushed and sieved with an average particle size of 320 microns, and the products are aseptically vacuum packaged and stored in storage.

[0067] The slant culture refers to inoculating the expanded cultured strains in a liquid culture solution, shaking culture on a shaker at 150 rpm and shaki...

Embodiment 3

[0080] Use the γ-linolenic acid bacteria that exist in some spore plants and seed vegetable oils as the culture and fermentation bacteria:

[0081] Screen the strains in the incubator;

[0082] The slant culture of the strains is vibrated for 2 days at 32℃;

[0083] Fermented for 1 day at 32℃ in the first-level fermentation tank;

[0084] Fermented for 2 days at 30℃ in the secondary fermenter:

[0085] Fermented for 1 day at 32℃ in a tertiary fermentation tank;

[0086] Fermented in a four-stage fermentation tank at 30°C for 2 days;

[0087] After that, it was vibrated and dried under the conditions of 30°C and 0.4 Pascals, or was blow-dried in a vibration mode with air with a humidity of 30% at -20°C.

[0088] Finally, it is crushed and sieved with an average particle size of 216 microns, and the product is aseptically vacuum packaged and stored.

[0089] The slant culture means that the expanded cultured strains are inoculated in a liquid culture solution, and shake cultured on ...

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Abstract

The invention discloses a process for producing dry bacterial powder which contains gamma-linolenic acid, comprising the following steps of screening strain, inclined surface vibrating culture, multi-stage fermentation, filtering, dehydration, drying, crushing and packaging. Further, the improvement of the invention is that the dying procedure is realized under the condition of -40 to 30 DEG C. The specific process includes that the vibrating drying is proceeded on the condition of 20-30 DEG C and 0.4-0.6 Pascal, or the drying is proceeded by utilizing air of which the moisture degree is 20% to 30% in the manner of vibration under the condition of -40 to 20 DEG C. The invention ensures the survival rate of bacterium through improving the drying art, thereby guaranteeing the quality of gamma-linolenic acid, and improving the quality of the final product. The invention also provides the specific art parameter of each procedure and is capable of further ensuring the achievement of fine-quality products.

Description

Technical field [0001] The invention relates to a method for producing dry bacteria powder, and more specifically, to an improvement in the process for producing dry bacteria powder containing γ-linolenic acid. Background technique [0002] The Chinese invention patent with the application number 03117250.4 filed on January 27, 2003 "The fermentation production process of dry bacterial powder containing γ-linolenic acid" and the Chinese invention patent with the application number 200410040053.2 filed on June 24, 2004" "New technology for producing dry bacterial powder containing γ-linolenic acid" respectively discloses methods for producing dry bacterial powder containing γ-linolenic acid. In both methods, the drying step is performed at 50-70°C. The problem is that at this temperature, the survival rate of bacteria is poor, especially γ-linolenic acid is prone to deterioration, which affects the quality of the final product. Summary of the invention [0003] In view of the abo...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/14C12N1/20A61K36/06A61K35/74A61K31/202A61K9/14A61P37/04A61P35/00A61P31/18A61P13/08A61P3/10A61P3/06A61P3/04A61P1/04
Inventor 崔亦鸿李忠云杨易
Owner 崔亦鸿
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