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Echelon circulation inoculation temperature-control compost method

A composting and step-by-step technology, applied in the preparation of organic fertilizers, organic fertilizers, fertilization devices, etc., can solve the problems of poor pertinence, loss of biological resources, and inability to promote the decomposition of other substances, and achieve the effect of reducing composting costs

Active Publication Date: 2008-05-28
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Since the above-mentioned methods all adopt the one-time input method when adding degrading bacteria or enzyme preparations to compost, there are the following disadvantages in composting one-time input of degrading bacteria or enzyme preparations: as adding a single enzyme preparation or single-function bacteria agent is often only beneficial to composting The degradation of a certain type of substance in the material cannot promote the decomposition of other substances in the pile; when the compound bacterial agent mixed with various microorganisms is used in one-time input method, the pertinence is not strong, and the microbial community after inoculation needs to be in different composts. Self-adjustment of the structure in the first stage will lose a lot of beneficial biological resources, and most of the bacteria with fiber degradation function are some high-temperature or medium-temperature fungi and actinomycetes, which often produce some secondary metabolism that restricts the growth of other bacteria product; the compound enzyme solution is inoculated once, and various enzyme systems often cannot play a role under the optimal reaction conditions, which wastes a lot of additive resources
However, these methods require the use of new bacterial agents or enzyme preparations for each batch fermentation, which increases the cost of composting, and the types and timing of adding bacterial agents or enzyme preparations need to be further studied and improved.

Method used

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  • Echelon circulation inoculation temperature-control compost method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of three different decay-promoting and degrading bacterial agents

[0025] Commercially purchased or obtained the required strains from the relevant microbiological depository institutions, on the corresponding culture medium, the slant activation culture and liquid expansion culture were carried out as usual.

[0026] The slant activation medium used includes PDA medium, nutrient agar medium, Ashubei medium, MRS medium, etc.;

[0027] The liquid expansion culture medium used includes PDA medium (for cultivating mold and yeast), broth medium (for cultivating bacteria), Gaoshi No. 1 medium (for cultivating actinomycetes), MRS medium (for cultivating lactic acid bacteria), etc. .

[0028] After the liquid expansion of different strains is completed, the weight ratio of various microbial culture solutions is directly used as follows:

[0029] Lipolytic bacteria 15~25%

[0030] Amyloliquefaciens 15~25%

[003...

Embodiment 2

[0063] The organic waste raw material consisting of 80% (weight percentage) of cow dung from dairy farms and 20% (weight percentage) of wheat straw is piled up in the form of piles, and the moisture content is controlled at 60% in the initial stage of fermentation.

[0064] a. In the early stage of composting, add the medium and low temperature decay-promoting and degrading bacterial agent directly according to 2% of the weight of the composting raw materials, and carry out composting and fermentation after fully mixing;

[0065] b. When the compost temperature rises to 55-60°C and enters the composting high-temperature period for more than 5 days, directly add thermophilic decay-promoting and degrading bacterial agents according to 2% of the weight of composting raw materials, and continue composting and fermentation after fully mixing;

[0066] c. After entering the compost cooling stage and the compost temperature drops to 30-40°C, add hemicellulose, cellulose, and lignin de...

Embodiment 3

[0077] As described in Example 2, in three different composting stages, during the composting and fermentation process of each stage, a part of the raw materials were taken out as functional bacteria agents for the next round of composting inoculation. The circulating inoculation amount is controlled at 10-20% (percentage by weight). After cyclic reinoculation, there is no need to add new strains for each fermentation, which not only significantly reduces the cost of composting, but also after multiple cyclic reinoculations, due to the more balanced and optimized functional flora, the composting effect is better.

[0078] Table 3 and Table 4 are the actual measurement results of various data after 10 rounds of echelon cycle reinoculation.

[0079] Table 3 The number of microorganisms in the compost after step cycle inoculation

[0080] (unit: cfu / g dry sample)

[0081] Before the second vaccination

[0082] Decomposition effect (1...

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Abstract

The invention relates to a method of temperature controlled compost for echelon circulation inoculation. Among three different stages in the composting process, three different degradation facilitating microbial inocula are added respectively according to composting temperature. In each stage of fermentation and compost, a part of raw materials are taken out to serve as the functional microbial inoculum which is used as composting inoculation next time. Since a new microbial inoculum is unnecessary to be inoculated in each fermentation process, compost cost is obviously reduced and circulation inoculation can effectively overcome the antagonism problem between external connection microorganism and indigenous microorganism, thus fully developing the positive effect of the indigenous microorganism and achieving the effect of accomplishing twice the result with half the effort.

Description

1. Technical field [0001] The invention belongs to the technical field of using microbial fermentation to produce natural fertilizers in agricultural science, and in particular relates to a method for inoculating microorganisms in stages and controlling the temperature of the compost used for composting and fermenting agricultural organic waste. 2. Background technology [0002] my country is a large agricultural country, which produces about 5 billion tons of agricultural waste every year, including livestock and poultry manure, crop stalks, township domestic waste, processing industry waste, etc. The random discarding of these organic wastes not only causes serious environmental problems, It is also a huge waste of resources. [0003] Aerobic composting is the most widely used composting method at present. The traditional aerobic composting method mainly utilizes the natural fermentation of indigenous microorganisms in the composting materials. Various microorganisms act a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00
CPCY02W30/40
Inventor 王一明林先贵
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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