HLH transcription control factor BmHLH1 for cultivated silk gland specification expression

A transcriptional regulator, silk technology, applied in genetic engineering, plant gene improvement, animal/human peptides, etc., can solve the problem of not finding regulatory factors, so as to promote the development of silk industry, increase silk production, and improve silk quality Effect

Inactive Publication Date: 2008-03-12
SOUTHWEST UNIVERSITY
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Problems solved by technology

The number of silk gland cells and the number of nucleus replication in silkworm are closely related to the silk production of silkworm. However, there are still relatively few theoretical studies on the production of silk gland cells in silkworm and the replication and division of nuclei, and no related regulatory factors have been found yet.

Method used

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Embodiment

[0043] Through the information analysis of the silkworm gene chip, it was found that the gene corresponding to the probe number sw15083 was specifically expressed in the silk gland of the silkworm, and the coded protein number of the corresponding silkworm was Bmb030021, and the gene was named BmHLH1; its protein sequence was in the SMART domain prediction software The structural domain predictions showed that they were proteins containing HLH domains; the BLAST program was run in NCBI to search for homologous genes, and the search results showed that the BmHLH1 protein had the highest similarity with the transcription factor Sage specifically expressed in the salivary glands of Drosophila, and its value was E=1e-24; analysis results show that this transcription factor specifically expressed in silk gland is a new type of bHLH transcriptional regulator specifically expressed in silk gland of silkworm; according to the functional research of its homologous genes, it shows that th...

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Abstract

The invention provides a HLH transcription and adjusting factor BmHLH1 for silk glands of silkworms, which is obtained by a series of biologic cloning technology such as analysis on the expression spectrum of silk-gland gene, making gene chip, hybridizing and chip-data analysis, etc. The HLH transcription and adjusting factor got by the invention is a novel transcription and adjusting factor for special expression of silkworms' silk glands, which is of important connection with the growth of silk glands and differentiation of silk-gland cells according to fluorescent quantitative PCR experiment; and HLH transcription factor is of important adjusting function for cell differentiation, organ specialization and special expression of gene according to interference study on BmHLH1. These research achievements are of important meaning for improving the silk output of silkworms, improving silk quality and promoting the silk industry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a transcription regulation factor specifically expressed from the silk gland of silkworm silkworm. Background technique [0002] The silk gland is the only organ of the silkworm that can produce cocoon silk, and it is the biological basis of the entire silk industry. A silkworm usually grows and develops for about 25 days. In the larval stage, it eats about 22g of fresh mulberry leaves and synthesizes and secretes 0.5-1g of silk. Protein, especially in the middle and late stages of the 5th instar, silkworms can convert mulberry leaf protein into silk protein with amazing efficiency. Its protein synthesis ability and efficiency are the strongest and highest among all biological tissues and organs discovered so far. According to the preliminary speculation of protein synthesis, this high-efficiency protein synthesis not only requires the coordination of many genes, but also the expres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435
Inventor 赵萍刘春徐汉福夏庆友向仲怀
Owner SOUTHWEST UNIVERSITY
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