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Process for producing single-cell protein by employing ocean saccharomycete

A single-cell protein and yeast technology, applied in the field of yeast production of single-cell protein, can solve problems such as unseen single-cell protein, and achieve the effects of wide carbon source growth range, low production cost and high protein content

Inactive Publication Date: 2008-03-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, there is no technology to produce single-cell protein rich in protein and other nutrients by using marine yeast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] 1. Preparation of production medium

[0009] ①Preparation of Jerusalem artichoke extract: Weigh fresh Jerusalem artichoke washed and sliced, add cold aged sea water, bath in 80 ℃ water for 15 minutes, and then cool, the resulting slurry is filtered with 4 layers of gauze, and the filtrate is diluted with aged sea water to the weight percentage of Jerusalem artichoke The content is 2-8%.

[0010] ②Preparation of soybean cake hydrolysate: Weigh 32g of soybean cake powder and 250ml 0.25N hydrochloric acid (tap water configuration), and sterilize it under 14 pounds of pressure for 25 minutes. After cooling, adjust the pH of the resulting mixture to 5.5- with 1N NaOH aqueous solution. 7.0, filter the supernatant and dilute it with tap water until the weight percentage of the soybean meal powder is 1.0%-5.0%.

[0011] ③ Preparation of production medium: The production medium is prepared by the above-mentioned Jerusalem artichoke extract and soybean cake hydrolysate in a weight ra...

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PUM

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Abstract

The technological process of utilizing marine yeast in producing single cell protein features that marine yeast G7a is inoculated to and cultured in the production culture medium prepared with Jerusalem artichoke extracting solution and soybean residue hydrolysate. The marine yeast of the present invention is marine yeast G7a in the preservation number of CGMCC No. 2006 and with very high protein content and wide growing carbon source range. The marine yeast G7a can secrete great amount of extracellular inulase and may be cultured in large scale to prepare single cell protein. The single cell protein with high protein content, essential amino acid content and especially content of lysine and leucine capable of promoting marine fish growth in 12.6 % and 9.1 % separately may be produced in low cost.

Description

Technical field [0001] The invention relates to a process for producing single-cell protein using yeast, in particular to a process for using marine yeast to produce single-cell protein from Jerusalem artichoke extract. Background technique [0002] In the past 20 years, China's marine aquaculture industry has developed rapidly, so the demand for bait for marine aquaculture animals has also grown steadily. The current marine aquaculture industry in my country widely uses some fish meat and microalgae such as spirulina, Chlorella and brown algae as bait. The microalgae that reproduce in natural waters are natural bait for aquatic animals. They have high protein content, good amino acid composition, and contain Chlorophyll and carotenoids and other pigments, but due to environmental pollution and lack of biological resources, in recent years, the harvest of this kind of bait has been difficult, the production cost has increased, and the digestibility is low and the palatability is ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P21/00
Inventor 李静池振明王祥红高玲美
Owner OCEAN UNIV OF CHINA
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