Application of recombinant adenovirus in producing antineoplastic medicine

Abstract

The invention discloses an application of recombinant adenovirus of human-carrying WWOX gene to prepare antineoplastic drug in the molecular biological domain, which is characterized by the following: constructing recombinant adenovirus of human-carrying WWOX gene; establishing drug tolerant model A549 Paclitaxel drug-tolerant strain and gene transmission method; observing the expression of adenovirus in the A549 / T cell; reversing the expression of Paclitaxel and cisplatin through WWOX in the external drug sensitive test. The lung cancer is malignant tumour with highest mortality based on general chemotherapy as main therapeutic method, but most of NSCLC displays insensitivity corresponding to chemotherapy in vivo and in vitro. The invention can reverse the drug tolerance of lung cancer cell, which provides new method to solve the drug tolerant problem of malignant tumour.

Description

technical field [0001] The invention relates to the field of biology, in particular to the application of a recombinant adenovirus carrying human WWOX gene in the preparation of antitumor drugs, in particular to the preparation of chemotherapeutic drug sensitizers. Background technique [0002] Lung cancer is a malignant tumor with a high mortality rate. The main reasons are high incidence, low possibility of surgical cure and poor response to chemotherapy. 80% of lung cancer patients are non-small cell lung cancer, and about 40% of non-small cell lung cancer is diagnosed at an advanced stage, systemic chemotherapy has become the main treatment. But we have to face the fact that the vast majority of non-small cell lung cancers (NSCLC) have been shown to be chemotherapy insensitive both in vivo and in vitro. [0003] One of the biggest obstacles in tumor chemotherapy is the resistance of tumor cells to drugs. Some tumors, such as colorectal cancer cells, have inherent resis...

AI Technical Summary

Problems solved by technology

[0004] The multidrug resistance characteristics of tumors can be summarized into four types: generally including one, insufficient effective drug concentration in cells: (1) insufficient drug intake; (2) drug pumping: chemotherapy absorbed by cells If the drug is re-pumped before it takes effect, the drug effect cannot be exerted, which is another reason for the insufficient concentration of the effective drug in the cell

(3) Intracellular drugs are "blocked" so that they cannot reach the target, resulting in a relative shortage of drugs

3. Drug resistance through DNA repair pathway: The DNA repair function of tumor cells can antagonize the DNA toxicity of chemotherapy drugs, resulting in drug resistance

The currently discovered MDR reversal agents are far from meeting the above requirements, and most of the currently used RMs are highly toxic, and it is difficult to reach an effective concentration in vitro, so the clinical effect is not ideal

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