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Egg loosening liquid for shrimp with eggs and method for quick egg-loosening by using the same

A fast technology for brooding shrimp, which is applied in the field of rapid de-egging, achieves obvious effects, complete de-egging, and promotes industrialization

Inactive Publication Date: 2008-02-27
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the egg-bearing female shrimp cannot be directly used for cross-breeding, and the eggs must be shed before it can be mated with the male shrimp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Select 25 kg of egg-bearing female shrimp with no disease, no disability, and good vitality, with a specification of 400 tails / kg, and raise them in cages for standby; use a cement pool with a length of 2 meters × width of 1 meter × height of 1 meter as the de-egg pool , the bottom of the pool is equipped with 25 evenly distributed inflatable heads, and there is a water outlet at the lowest center of the bottom of the pool. Put the deionized water into the egg removal tank, first add 6 kg of chemically pure sodium chloride, and stir until it is completely dissolved; then add 2 kg of trypsin with a specific activity of 1:220, and stir until it is completely dissolved; Minutes later, put in 25 kg of egg-bearing shrimps, keep the water temperature between 18°C ​​during the de-egging process, and keep aerating continuously to increase oxygen. , the de-egging time is 3.5 hours; after the de-egging is completed, fish out the female shrimp with a net and put it in the pond for...

Embodiment 2

[0034] Adopt 50 kilograms of egg-bearing shrimps, the specification is 350 tails / kg, and the water temperature is 24°C. Add 7 kilograms of chemically pure sodium chloride to 1000 kilograms of deionized water, and 2.5 kilograms of pepsin with an activity of 150,000 units, and adopt the same method as in Example 1. The egg removal method is carried out, and the egg removal temperature is 24°C. The ovulation time is 2.5 hours, and the survival rate of female shrimp is 95%.

Embodiment 3

[0036] Adopt 50 kilograms of brood shrimp, specification is 350 tails / kg, water temperature is 22 ℃, add chemically pure sodium chloride 7 kilograms in 1000 kilograms of deionized waters, the activity is 2.5 kilograms of alkaline protease of 100,000 units, and adopt embodiment 1 The same method is used for deovulation, and the deovulation temperature is 22°C. The ovulation time is 3 hours, and the survival rate of female shrimp is 95%.

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PUM

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Abstract

The invention discloses a deegging liquid and method to strip egg in the abdominal region of hen rapidly without injuring, wherein the deegging liquid contains proteinase and sodium chloride at certain proportion. The deegging method comprises the following steps: placing the incubated hen into deegging liquid to enzymolyze; aerating continuously during enzymolytic course; stripping the whole egg from abdominal region of hen completely; finishing the deegging course within 5h; maintaining the survival rate of deegged hen and egg over 90%. The invention has first break-through of rapid and non-injured deegging of the incubating hen, which can be applied to crossbreed, do manufactured breeding, collect and manufacture fresh water shrimp egg.

Description

technical field [0001] The invention relates to shrimp deovulation technology, in particular to a deovulation solution for rapidly and non-damaging removal of abdominal eggs of ovum-bearing female shrimps by applying enzymatic hydrolysis reaction and a method for rapid deovulation using the same. Background technique [0002] Freshwater shrimp, scientific name Macrobrachium japonica, has tender meat, delicious taste, short breeding cycle, fast growth and high economic value. It is an important cultured shrimp in my country. As the largest province of freshwater shrimp farming in my country, Jiangsu Province has an annual breeding scale of nearly 2 million mu, with an annual breeding output of 60,000-80,000 tons and an annual output value of about 3 billion yuan; Zhejiang, Shanghai, Anhui and other provinces and cities, freshwater shrimp It has also become one of the leading varieties of aquaculture; in recent years, the culture of freshwater shrimp in many provinces and regio...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K67/00A61D19/00
CPCY02A40/81
Inventor 傅洪拓龚永生吴滟熊贻伟何新龙
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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